Solution structure of an antifreeze protein CfAFP-501 from Choristoneura   fumiferana

doi:10.1007/s10858-005-8206-3

CORC > 北京大学 > 化学与分子工程学院

	Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana
	Li, CM ; Guo, XR ; Jia, ZC ; Xia, B ; Jin, CW
刊名	生物分子核磁共振杂志
	2005
关键词	antifreeze protein constrains dynamics Nuclear Magnetic Resonance solution structure SPRUCE BUDWORM CHEMICAL-SHIFT NMR DYNAMICS PROGRAM C-13 ISOFORM
DOI	10.1007/s10858-005-8206-3
英文摘要	Antifreeze proteins (AFPs) are widely employed by various organisms as part of their overwintering survival strategy. AFPs have the unique ability to suppress the freezing point of aqueous solution and inhibit ice recrystallization through binding to the ice seed crystals and restricting their growth. The solution structure of CfAFP-501 from spruce budworm has been determined by NMR spectroscopy. Our result demonstrates that CfAFP-501 retains its rigid and highly regular structure in solution. Overall, the solution structure is similar to the crystal structure except the N- and C-terminal regions. NMR spin-relaxation experiments further indicate the overall rigidity of the protein and identify a collection of residues with greater flexibilities. Furthermore, Pro91 shows a cis conformation in solution instead of the trans conformation determined in the crystal structure.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000231601300007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Spectroscopy; SCI(E); 6; ARTICLE; 3; 251-256; 32
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/253303]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, CM,Guo, XR,Jia, ZC,et al. Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana[J]. 生物分子核磁共振杂志,2005.
APA	Li, CM,Guo, XR,Jia, ZC,Xia, B,&Jin, CW.(2005).Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana.生物分子核磁共振杂志.
MLA	Li, CM,et al."Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana".生物分子核磁共振杂志 (2005).