Separation and detection of vitellogenin in fish plasma by capillary   zone electrophoresis

doi:10.1016/j.jchromb.2005.04.013

CORC > 北京大学 > 化学与分子工程学院

	Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis
	Song, MY ; Wang, JN ; Shao, J ; He, B ; Jiang, GB ; Shi, GQ
刊名	journal of chromatography b analytical technologies in the biomedical and life sciences
	2005
关键词	capillary zone electrophoresis vitellogenin fish plasma LINKED-IMMUNOSORBENT-ASSAY CHROMATOGRAPHIC METHOD ENDOCRINE DISRUPTION PIMEPHALES-PROMELAS PURIFICATION SERUM BIOMARKER PROTEINS ELISA INDUCTION
DOI	10.1016/j.jchromb.2005.04.013
英文摘要	A method for coupling capillary zone electrophoresis (CZE) with rapid membrane chromatography purification (RMCP) was established for the analysis of vitellogenin (VTG) in male fish plasma induced with 17 beta-estrodiol. CZE analyses of purified VTG were performed in a buffer containing 25 mM sodium borate (pH 8.4). A 50 mu m i.d. fused-silica capillary was used for separation and the detection was carried out by UV-diode array at 214 nm. Inter- and intra-assay variabilities of the proposed method were less than 10.06 and 1.95%, respectively. The method has good linear relationship over the scope of 15-2250 mu g/ml with a correlation coefficient of R-2 = 0.9965 and a detection limit of 7.0 mu g/ml. The established CZE method was also applied to directly separate and identify VTG from fish plasma. The results indicated this method could minimize interferences from plasma proteins, allowing the detection of at least 62.5 mu g/ml of VTG proteins in total proteins. This is a rapid and easy method to determine the quantity and purity of VTG compared to Bradford method and SDS-PAGE. (c) 2005 Elsevier B.V. All rights reserved.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; 2; ARTICLE; 1; 38-44; 821
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/253266]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Song, MY,Wang, JN,Shao, J,et al. Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis[J]. journal of chromatography b analytical technologies in the biomedical and life sciences,2005.
APA	Song, MY,Wang, JN,Shao, J,He, B,Jiang, GB,&Shi, GQ.(2005).Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis.journal of chromatography b analytical technologies in the biomedical and life sciences.
MLA	Song, MY,et al."Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis".journal of chromatography b analytical technologies in the biomedical and life sciences (2005).