蛋白质微阵列SPR实时相位检测

doi:10.3866/PKU.WHXB20050813

CORC > 北京大学 > 化学与分子工程学院

	蛋白质微阵列SPR实时相位检测; Real-time SPR Phase Detection for Protein Micro-array
	余兴龙 ; 魏星 ; 王鼎新 ; 定翔 ; 廖玮 ; 赵新生
刊名	物理化学学报
	2005
关键词	蛋白质微阵列相位检测偏振光干涉 SPR protein micro-array SPR phase detection interference of polarized light
DOI	10.3866/PKU.WHXB20050813
英文摘要	将表面等离子体共振(surface plasmon resonance,SPR)与空间相位调制检测结合,用一束准直的平行光照射传感芯片,在反射光路中引入渥拉斯顿棱镜,使光束中的p光和s光产生偏振光干涉形成条纹,生化反应的有关信息从干涉条纹的相位变化中得出.选择金膜厚度为30 nm和40 nm的两种芯片,分别用浓度为50%和20%的酒精-水溶液进行实验,得到SPR阵列图谱;选择金膜厚度为30 nm,兔IgG作为阳性对照,牛血清白蛋白(BSA)作为阴性对照,与0.2 mL的羊抗兔IgG、1.8 mL水的混合溶液反应,得到阵列反应图谱.实验结果表明,这种方法具有抗干扰能力强,灵敏度高,无需标记和可实时检测等优点,能满足蛋白质芯片检测的要求.; Combining surface plasmon resonance (SPR) and spatial phase modulation, a real-time SPR sensing chip was illuminated with a collimated parallel light beam. A Wollaston prism was introduced into the detection light path, causing the p- and s-polarization components to interfere with each other. The information of biochemical reactions could be obtained by extracting the phase change from the interference patterns. The sensor chips, on which the Au film was 30 mn and 40 nm, experimented with 50% and 20% alcohol solutions respectively to obtain SPR array graph. Subsequently, two proteins, rabbit IgG as positive sample and Bovine Serum Albumin (BSA) as negative control, reacted with the mixed solution of 0.2 mL goat-anti-rabbit IgG sample and 1.8 mL deionized water, using Au film of 30 nm. The reaction diagram was also attained. The experimental results indicated that, with the merits of high noise resistance, high sensitivity, unlabelled and real-time detection, this SPR method could meet the requirement of protein micro-array detection.; 国家自然科学基金; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000231293900014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; 8; 888-892; 21
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/253137]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	余兴龙,魏星,王鼎新,等. 蛋白质微阵列SPR实时相位检测, Real-time SPR Phase Detection for Protein Micro-array[J]. 物理化学学报,2005.
APA	余兴龙,魏星,王鼎新,定翔,廖玮,&赵新生.(2005).蛋白质微阵列SPR实时相位检测.物理化学学报.
MLA	余兴龙,et al."蛋白质微阵列SPR实时相位检测".物理化学学报 (2005).