A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles | |
Liu, Xiaoyan ; Ren, Xiufang ; Deng, Xiaoyong ; Huo, Yinan ; Xie, Jiang ; Huang, Hai ; Jiao, Zheng ; Wu, Minghong ; Liu, Yuanfang ; Wen, Tieqiao | |
刊名 | biomaterials |
2010 | |
关键词 | Titanium dioxide nanoparticles Neural stem cells Differentiation Protein-protein interaction Proteomics HUMAN DERMAL FIBROBLASTS EPITHELIAL-CELLS PROGENITOR CELLS IN-VITRO GENE-EXPRESSION RAT ACTIVATION RECEPTORS EPSILON NEUROPROTECTION |
DOI | 10.1016/j.biomaterials.2009.12.054 |
英文摘要 | The effects of titanium dioxide (TiO(2)) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO(2) NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO(2) NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO(2) NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphotylation, which is negatively regulated by the protein kinase C epsilon (PKC epsilon), is demonstrated. It is inferred that PKCE plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO(2) NPs exposure. (C) 2009 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000275946500013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; 21; ARTICLE; 11; 3063-3070; 31 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/243974] |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Liu, Xiaoyan,Ren, Xiufang,Deng, Xiaoyong,et al. A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles[J]. biomaterials,2010. |
APA | Liu, Xiaoyan.,Ren, Xiufang.,Deng, Xiaoyong.,Huo, Yinan.,Xie, Jiang.,...&Wen, Tieqiao.(2010).A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles.biomaterials. |
MLA | Liu, Xiaoyan,et al."A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles".biomaterials (2010). |
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