Dual Molecular Light Switches for pH and DNA Based on a Novel Ru(II)   Complex. A Non-Intercalating Ru(II) Complex for DNA Molecular Light   Switch

doi:10.1021/ic102126m

CORC > 北京大学 > 化学与分子工程学院

	Dual Molecular Light Switches for pH and DNA Based on a Novel Ru(II) Complex. A Non-Intercalating Ru(II) Complex for DNA Molecular Light Switch
	Zhang, An-Guo ; Zhang, You-Zhuan ; Duan, Zhi-Ming ; Wang, Ke-Zhi ; Wei, Hui-Bo ; Bian, Zu-Qiang ; Huang, Chun-Hui
刊名	inorganic chemistry
	2011
关键词	EFFECTIVE CORE POTENTIALS BINDING AFFINITY ETHIDIUM-BROMIDE RUTHENIUM(II) COMPLEX METAL-COMPLEXES ACID-BASE DIPYRIDOPHENAZINE COMPLEXES PHOTOPHYSICAL PROPERTIES DEOXYRIBONUCLEIC-ACID SEQUENCE SELECTIVITY
DOI	10.1021/ic102126m
英文摘要	A new Ru(II) complex of [Ru(phen)(2)(Hcdpq)](ClO(4))(2) {phen = 1,10-phenanthroline, Hcdpq = 2-carboxyl-dipyrido[3,2-f:2',3'-h]quinoxaline} was synthesized and characterized. The spectrophotometric pH and calf thymus DNA (ct-DNA) titrations showed that the complex acted as a dual molecular light switch for pH and ct-DNA with emission enhancement factors of 17 and 26, respectively. It was shown to be capable of distinguishing ct-DNA from yeast RNA with this binding selectivity being superior to two well-known DNA molecular light switches of [Ru(bpy)(2)(dppz)](2+) {bpy =2,2'-bipyridine, and dppz = dipyrido-[3,2-a:2',3'-c]phenazine} and ethidium bromide. The complex bond to ct-DNA probably in groove mode with a binding constant of (4.67 +/- 0.06) x 10(3) M(-1) in 5 mM Tris-HCl, 50 mM NaCl (pH = 7.10) buffer solution, as evidenced by UV-visible absorption and luminescence titrations, the dependence of DNA binding constants on NaCl concentrations, DNA competitive binding with ethidium bromide, and emission lifetime and viscosity measurements. To get insight into the light-switch mechanism, theoretical calculations were also performed by applying density functional theory (DFT) and time-dependent DFT.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292618000007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Inorganic & Nuclear; SCI(E); 45; ARTICLE; 14; 6425-6436; 50
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/238789]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, An-Guo,Zhang, You-Zhuan,Duan, Zhi-Ming,et al. Dual Molecular Light Switches for pH and DNA Based on a Novel Ru(II) Complex. A Non-Intercalating Ru(II) Complex for DNA Molecular Light Switch[J]. inorganic chemistry,2011.
APA	Zhang, An-Guo.,Zhang, You-Zhuan.,Duan, Zhi-Ming.,Wang, Ke-Zhi.,Wei, Hui-Bo.,...&Huang, Chun-Hui.(2011).Dual Molecular Light Switches for pH and DNA Based on a Novel Ru(II) Complex. A Non-Intercalating Ru(II) Complex for DNA Molecular Light Switch.inorganic chemistry.
MLA	Zhang, An-Guo,et al."Dual Molecular Light Switches for pH and DNA Based on a Novel Ru(II) Complex. A Non-Intercalating Ru(II) Complex for DNA Molecular Light Switch".inorganic chemistry (2011).