Fast Screening for Tobacco-Specific N-nitrosamines by CZE Using   Dynamically Coated Capillaries

doi:10.1007/s10337-011-2086-3

CORC > 北京大学 > 化学与分子工程学院

	Fast Screening for Tobacco-Specific N-nitrosamines by CZE Using Dynamically Coated Capillaries
	Liao, Jie ; Pan, Yinjin ; Li, Chenchen ; Wen, Dawei ; Liu, Huwei
刊名	chromatographia
	2011
关键词	Capillary zone electrophoresis Dynamic coating Screening Tobacco-specific nitrosamines CARBOHYDRATE-DEFICIENT TRANSFERRIN SOLID-PHASE EXTRACTION ZONE-ELECTROPHORESIS MASS-SPECTROMETRY IDENTIFICATION ELECTROSPRAY RABBIT SERUM DNA
DOI	10.1007/s10337-011-2086-3
英文摘要	In this paper, we propose a rapid capillary zone electrophoretic method with dynamically coated capillaries for separation of and screening for six tobacco-specific nitrosamines, a group of strong carcinogens found only in tobacco products. Dynamic coating of the capillary surface with a polycation coating (CElixir Reagent A) followed by a polyanion coating (CElixir Reagent B; pH 2.5) was accomplished by use of a commercially available reagent kit. With this method, six tobacco-specific nitrosamines: N'-nitrosonornicotine, N'-nitrosoanatabine, N'-nitrosoanabasine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, and 4-(methylnitroamino)-4-(3-pyridyl)-1-butanol were simultaneously separated and determined within 3 min, with a satisfactory detection limit of 1.0 mu g mL(-1) for all the tobacco-specific nitrosamines at S/N = 3, and a linear relationship between UV absorbance and analyte concentration (R (2) > 0.98). The repeatability of the method ranged from 0.24 to 0.73% (RSD, n = 5) for migration time and 0.25-1.54% (RSD, n = 5) for peak area, demonstrating that this capillary electrophoretic method is better than conventional capillary zone electrophoresis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294066000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 2; ARTICLE; 5-6; 415-419; 74
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/238610]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liao, Jie,Pan, Yinjin,Li, Chenchen,et al. Fast Screening for Tobacco-Specific N-nitrosamines by CZE Using Dynamically Coated Capillaries[J]. chromatographia,2011.
APA	Liao, Jie,Pan, Yinjin,Li, Chenchen,Wen, Dawei,&Liu, Huwei.(2011).Fast Screening for Tobacco-Specific N-nitrosamines by CZE Using Dynamically Coated Capillaries.chromatographia.
MLA	Liao, Jie,et al."Fast Screening for Tobacco-Specific N-nitrosamines by CZE Using Dynamically Coated Capillaries".chromatographia (2011).