In vitro studies on dissolved substance of cinnabar: Chemical species   and biological properties

doi:10.1016/j.jep.2010.06.018

CORC > 北京大学 > 化学与分子工程学院

	In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties
	Zhou, Xinrui ; Zeng, Kewu ; Wang, Qi ; Yang, Xiaoda ; Wang, Kui
刊名	journal of ethnopharmacology
	2010
关键词	Cinnabar Mercuric polysulfide Caco-2 monolayer Protein binding ATOMIC FLUORESCENCE SPECTROMETRY TRADITIONAL MEDICINES MERCURIAL COMPOUNDS PROXIMAL TUBULE CELL-CULTURE ABSORPTION SULFIDE PERMEABILITY MONOLAYERS SPECIATION
DOI	10.1016/j.jep.2010.06.018
英文摘要	Cinnabar is one of traditional Chinese medicines widely used in many Asian countries. It is also a medicine with potential toxicity especially when taking overdose. Up to date, studies on the mechanism of the biological activity of cinnabar were still insufficient. Aim of the study: To investigate the possible bioactive species from cinnabar after oral administration, which is the fundamental of biological effects of cinnabar. Materials and methods: Under mimetic intestinal and gastric conditions, the chemical components dissolved from cinnabar were analyzed by infrared spectroscopy (IR) and Raman spectroscopy. Furthermore, binding of mercuric species of cinnabar extractions to human serum protein (HSA) was characterized and their intestinal permeability was determined using the Caco-2 cell monolayer. The cytotoxicity of cinnabar extractions was assessed on human kidney-2 (HK-2) cell. Results: Major dissolved species included mercuric polysulfide (i.e. HgS(2)(OH)(-) and Hg(3)S(2)Cl(2)). The apparent permeability coefficient (P(app)) of mercuric polysulfides was (1.6 +/- 0.3) x 10(-6) cm/s, which is slightly lower than that of mercuric chloride (HgCl(2)). Unlike HgCl(2), mercuric polysulfides exhibited two tightly binding sites to HSA and had little effect on viability of HK-2 cells. Conclusion: Mercuric polysulfides, as the major dissolved components, may serve as the active species of cinnabar exhibiting pharmacological and/or toxicological effects. (C) 2010 Elsevier Ireland Ltd. All rights reserved.; Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy; SCI(E); PubMed; 12; ARTICLE; 1; 196-202; 131
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/195809]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhou, Xinrui,Zeng, Kewu,Wang, Qi,et al. In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties[J]. journal of ethnopharmacology,2010.
APA	Zhou, Xinrui,Zeng, Kewu,Wang, Qi,Yang, Xiaoda,&Wang, Kui.(2010).In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties.journal of ethnopharmacology.
MLA	Zhou, Xinrui,et al."In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties".journal of ethnopharmacology (2010).