A self-assemble aptamer fragment/target complex based high-throughput   colorimetric aptasensor using enzyme linked aptamer assay

doi:10.1016/j.talanta.2012.11.018

CORC > 北京大学 > 化学与分子工程学院

	A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay
	Nie, Ji ; Deng, Yan ; Deng, Qin-Pei ; Zhang, De-Wen ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	talanta
	2013
关键词	Enzyme linked aptamer assay Colorimetry Cocaine Small molecule Biomacromolecule GOLD NANOPARTICLES ELECTROCHEMICAL APTASENSOR FLUORESCENCE ANISOTROPY COCAINE DNA THROMBIN DNAZYME SENSOR AMPLIFICATION MOLECULES
DOI	10.1016/j.talanta.2012.11.018
英文摘要	Enzyme linked aptamer assay (ELAA) uses an aptamer as recognition element and enzyme as signal readout element for establishing different kinds of aptasensors. We reported herein a high-throughput colorimetric aptasensor based on ELAA only requiring a single aptamer sequence for cocaine detection. An anti-cocaine aptamer was cleaved into two fragments, one of which was immobilized on a DNA-BIND 96-well plate via 5'-labeled primary amine and the other one was biotin labeled. The presence of two aptamer fragments and the target molecule led to the formation of aptamer fragments/target complexes. Streptavidin-horseradish peroxidase (SA-HRP) was used to react with biotin in order to obtain quantitative signals. A linear response towards cocaine concentration in the range of 5-200 mu M and a detection limit down to 2.8 mu M (S/N=3) were achieved. The specificity and application in real sample were validated. Furthermore, a verification test of thrombin detection in the same strategy illustrated its feasibility for not only small molecule but also biomacromolecule. With the advantage of high-throughput, easy operation, high specificity, the colorimetric assay based on ELAA requiring a single aptamer sequence opens up a new approach for detecting different kinds of targets via specific affinity recognition among target and suitably cleaved aptamer fragments. (C) 2012 Elsevier B.V. All rights reserved.; Chemistry, Analytical; SCI(E); PubMed; 7; ARTICLE; 309-314; 106
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190542]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Nie, Ji,Deng, Yan,Deng, Qin-Pei,et al. A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay[J]. talanta,2013.
APA	Nie, Ji,Deng, Yan,Deng, Qin-Pei,Zhang, De-Wen,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2013).A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay.talanta.
MLA	Nie, Ji,et al."A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay".talanta (2013).