G-quadruplex based two-stage isothermal exponential amplification   reaction for label-free DNA colorimetric detection

doi:10.1016/j.bios.2014.01.032

CORC > 北京大学 > 化学与分子工程学院

	G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection
	Nie, Ji ; Zhang, De-Wen ; Tie, Cai ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	生物传感器与生物电子学
	2014
关键词	G-quadruplex DNA detection Isothermal exponential amplification Label-free Colorimetry HYBRIDIZATION CHAIN-REACTION AMPLIFIED DETECTION QUANTITATIVE DETECTION SENSITIVE DETECTION NUCLEIC-ACID MICRORNAS DNAZYME POLYMERASE PROBE
DOI	10.1016/j.bios.2014.01.032
英文摘要	A novel G-quadruplex based two-stage isothermal exponential amplification reaction (GQ-EXPAR) was developed for label-free DNA calorimetric detection in this work. The exponential amplified trigger DNA in the first stage can convert into G-quadruplex sequence EAD2 by a linear amplification circuit in the second stage. Created EAD2 can form G-quadruplex/hemin DNAzyme to act as a direct signal readout element. The GQ-EXPAR combines the exponential amplification of DNA sequence and the peroxidase-mimicking DNAzyme induced signal amplification, which achieves tandem dual-amplification. Taking advantages of isothermal incubation, this label-free homogeneous assay obviates the need of thermal cycling. As no complex synthesis or extra downstream operation is needed, the whole easy handling procedure can be finished in no more than 1 h. This assay allows the sensing of the model DNA with the limit of detection to be 2.5 pM. Moreover, it demonstrates good discrimination of mismatched sequences. The strategy has also been successfully implemented to sensitively detect Tay Sachs genetic disorder mutant. (C) 2014 Elsevier B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); PubMed; 9; ARTICLE; zhouyl@pku.edu.cn; zxx@pku.edu.cn; 237-242; 56
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190238]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Nie, Ji,Zhang, De-Wen,Tie, Cai,et al. G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection[J]. 生物传感器与生物电子学,2014.
APA	Nie, Ji,Zhang, De-Wen,Tie, Cai,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2014).G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection.生物传感器与生物电子学.
MLA	Nie, Ji,et al."G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection".生物传感器与生物电子学 (2014).