Novel Homogeneous Label-Free Electrochemical Aptasensor Based on   Functional DNA Hairpin for Target Detection

doi:10.1021/ac402295y

CORC > 北京大学 > 化学与分子工程学院

	Novel Homogeneous Label-Free Electrochemical Aptasensor Based on Functional DNA Hairpin for Target Detection
	Zhang, De-Wen ; Nie, Ji ; Zhang, Fang-Ting ; Xu, Li ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	analytical chemistry
	2013
关键词	APTAMER-BASED SENSORS PEROXIDASE-ACTIVITY IMPEDANCE SPECTROSCOPY COLORIMETRIC DETECTION DNAZYME AMPLIFICATION BIOSENSOR COMPLEX PROTEIN ASSAY
DOI	10.1021/ac402295y
英文摘要	We first developed a label-free and immobilization-free homogeneous electrochemical aptasensor, which combined a smart functional DNA hairpin and a designed miniaturized electrochemical device. Cocaine was chosen as a model target. The anticocaine aptamer and peroxidase-mimicking DNAzyme were integrated into one single-stranded DNA hairpin. Both aptamer and G-quadruplex were elaborately blocked by the stem region. The conformation switching induced by the affinity interaction between aptamer and cocaine released G-quadruplex part and turned on DNAzyme activity. The designed electrochemical device, constructed by a disposable micropipet tip and a reproducible carbon fiber ultramicroelectrode, was applied to the detection of homogeneous DNAzyme catalytic activity at the microliter level. The aptasensor realized the quantification of cocaine ranging from 1 to 500 mu M with high specificity. The clever combination of the functional DNA hairpin and the novel device achieved an absolutely label-free electrochemical aptasensor, which showed excellent performance like low cost, easy operation, rapid detection, and high repeatability.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000330017500067&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); PubMed; 30; ARTICLE; 19; 9378-9382; 85
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189991]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, De-Wen,Nie, Ji,Zhang, Fang-Ting,et al. Novel Homogeneous Label-Free Electrochemical Aptasensor Based on Functional DNA Hairpin for Target Detection[J]. analytical chemistry,2013.
APA	Zhang, De-Wen,Nie, Ji,Zhang, Fang-Ting,Xu, Li,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2013).Novel Homogeneous Label-Free Electrochemical Aptasensor Based on Functional DNA Hairpin for Target Detection.analytical chemistry.
MLA	Zhang, De-Wen,et al."Novel Homogeneous Label-Free Electrochemical Aptasensor Based on Functional DNA Hairpin for Target Detection".analytical chemistry (2013).