Construction of antibody-like nanoparticles for selective protein sequestration in living cells

doi:10.1039/c4nr07615j

CORC > 北京大学 > 化学与分子工程学院

	Construction of antibody-like nanoparticles for selective protein sequestration in living cells
	Liu Yibin ; Fang Simin ; Zhai Junqiu ; Zhao Meiping
刊名	nanoscale
	2015
DOI	10.1039/c4nr07615j
英文摘要	We demonstrate the successful construction of fluorescently labeled magnetic antibody-like nanoparticles (ANPs) via a facile one-step surface-initiated in situ molecular imprinting approach over silica coated magnetite (Fe3O4@SiO2) core-shell nanocomposites. The as-prepared ANPs had a highly compact structure with an overall size of 83 ± 5 nm in diameter and showed excellent aqueous dispersion stability. With the predetermined high specificity to the target protein and high biocompatibility, the ANPs enabled rapid, efficient, selective and optically trackable sequestration of target proteins within living cells. This work represents the first example of fully artificially engineered multifunctional ANPs for the intracellular protein-sequestration without disruption of the cells. The established approach may be further extended to generate ANPs for various proteins of interest and provide useful tools for related biological research and biomedical applications.; SCI(E); EI; PubMed; 0; ARTICLE; mpzhao@pku.edu.cn; 16; 7162-7; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188190]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu Yibin,Fang Simin,Zhai Junqiu,et al. Construction of antibody-like nanoparticles for selective protein sequestration in living cells[J]. nanoscale,2015.
APA	Liu Yibin,Fang Simin,Zhai Junqiu,&Zhao Meiping.(2015).Construction of antibody-like nanoparticles for selective protein sequestration in living cells.nanoscale.
MLA	Liu Yibin,et al."Construction of antibody-like nanoparticles for selective protein sequestration in living cells".nanoscale (2015).