Oligonucleotides-based biosensors with high sensitivity and selectivity   for mercury using electrochemical impedance spectroscopy

doi:10.1016/j.elecom.2009.07.029

CORC > 北京大学 > 化学与分子工程学院

	Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy
	Cao, Rui-Guo ; Zhu, Bin ; Li, Jingjian ; Xu, Dongsheng
刊名	电化学通讯
	2009
关键词	Lable-free DNA Biosensor Mercury Electrochemical impedance spectroscopy HEAVY-METAL IONS COLORIMETRIC DETECTION GOLD NANOPARTICLES AQUEOUS-SOLUTION HG-II DNA ELECTRODE TRANSDUCTION LEAD(II) SENSORS
DOI	10.1016/j.elecom.2009.07.029
英文摘要	A novel electrochemical biosensor with high sensitivity and selectivity for mercuric ion detection, based on DNA self-assembly electrode, is designed. Thiol functionalized poly-T oligonucleotides were used as gold electrode modifier through formation of Au-S bond between DNA and gold electrode. In presence of Hg(2+) ions, the specific coordination between Hg(2+) and thymine bases can change parallel ss-DNA from linear to hairpin structures, which can cause the release of partial DNA molecules from the surface of the electrode. The density of DNA on the surface of electrode correlated with the concentration of mercury in the solution and can be monitored by electrochemical impedance spectroscopy. The limit of detection of this method is pM level of mercuric ions which is far below the upper limit of Hg(2+) mandated by United States Environmental Protection Agency (EPA), 2 ppb (10 nM). In addition, this method showed excellent selectivity. A series of divalent metal ions, including Ni(2+), Co(2+), Mg(2+), Zn(2+), Ba(2+) and Cd(2+), have little interference with the detection of Hg(2+). (C) 2009 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000270347500023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Electrochemistry; SCI(E); EI; 43; ARTICLE; 9; 1815-1818; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151409]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,et al. Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy[J]. 电化学通讯,2009.
APA	Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,&Xu, Dongsheng.(2009).Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy.电化学通讯.
MLA	Cao, Rui-Guo,et al."Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy".电化学通讯 (2009).