A distance-dependent metal-enhanced fluorescence sensing platform based   on molecular beacon design

doi:10.1016/j.bios.2013.09.013

CORC > 北京大学 > 化学与分子工程学院

	A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design
	Zhou, Zhenpeng ; Huang, Hongduan ; Chen, Yang ; Liu, Feng ; Huang, Cheng Zhi ; Li, Na
刊名	生物传感器与生物电子学
	2014
关键词	Metal-enhanced fluorescence Molecular beacon Distance-dependence Mercuric ion SINGLE-NUCLEOTIDE POLYMORPHISMS GOLD NANOPARTICLES DNA DETECTION SILVER NANOPARTICLES QUANTUM DOTS SENSOR MERCURY SURFACE HYBRIDIZATION PROBE
DOI	10.1016/j.bios.2013.09.013
英文摘要	A new metal-enhanced fluorescence (MEF) based platform was developed on the basis of distance-dependent fluorescence quenching-enhancement effect, which combined the easiness of Ag-thiol chemistry with the MEF property of noble-metal structures as well as the molecular beacon design. For the given sized AgNPs, the fluorescence enhancement factor was found to increase with a d(6) dependency in agreement with fluorescence resonance energy transfer mechanism at shorter distance and decrease with a d(-3) dependency in agreement with plasmonic enhancement mechanism at longer distance between the fluorophore and the AgNP surface. As a proof of concept, the platform was demonstrated by a sensitive detection of mercuric ions, using thymine-containing molecular beacon to tune silver nanoparticle (AgNP)-enhanced fluorescence. Mercuric ions were detected via formation of a thymine-mercuric-thymine structure to open the hairpin, facilitating fluorescence recovery and AgNP enhancement to yield a limit of detection of 1 nM, which is well below the U.S. Environmental Protection Agency regulation of the Maximum Contaminant Level Goal (10 nM) in drinking water. Since the AgNP functioned as not only a quencher to reduce the reagent blank signal but also an enhancement substrate to increase fluorescence of the open hairpin when target mercuric ions were present, the quenching-enhancement strategy can greatly improve the detection sensitivity and can in principle be a universal approach for various targets when combined with molecular beacon design. (C) 2013 Elsevier B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); 11; ARTICLE; lina@pku.edu.cn; 367-373; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150598]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhou, Zhenpeng,Huang, Hongduan,Chen, Yang,et al. A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design[J]. 生物传感器与生物电子学,2014.
APA	Zhou, Zhenpeng,Huang, Hongduan,Chen, Yang,Liu, Feng,Huang, Cheng Zhi,&Li, Na.(2014).A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design.生物传感器与生物电子学.
MLA	Zhou, Zhenpeng,et al."A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design".生物传感器与生物电子学 (2014).