Complexation of DNA with cationic surfactants as studied by small-angle   X-ray scattering

doi:10.1007/s11426-014-5159-y

CORC > 北京大学 > 化学与分子工程学院

	Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering
	Niu Lin ; Yan JingJing ; Yang XuYan ; Burger, Christian ; Rong LiXia ; Hsiao, Benjamin ; Liang DeHai
刊名	science china chemistry
	2014
关键词	phase behavior DNA surfactant synchrotron small-angle X-ray scattering kinetics GENE DELIVERY EFFICACIES TRANSGENE EXPRESSION SERUM COMPATIBILITY LIPOSOME COMPLEXES COLUMNAR PHASE LIPIDS TRANSFECTION NANOSTRUCTURE TRANSITION EFFICIENT
DOI	10.1007/s11426-014-5159-y
英文摘要	The phase behaviors of the complex formed by didodecyldimethylammonium bromide (DDAB) and cetyltrimethylammonium bromide (CTAB) interacting with three different types of DNAs, salmon testes DNA (similar to 2000 bp), 21-bp double-stranded oligonucleotides (oligo-dsDNA), and 21-nt single-stranded oligonucleotides (oligo-ssDNA) were studied by synchrotron small-angle X-ray scattering. It was found that the DNA length and flexibility, together with the positive/negative charge ratio, determined the final structure. At higher charge ratios, the DNA length exhibited negligible effect. Both oligo-dsDNA and salmon DNA formed inverted hexagonal packing of cylinders with CTAB, as well as bilayered lamella with DDAB. However, at lower charge ratios, oligo-dsDNA formed a distorted hexagonal phase with CTAB and a new structure with DDAB, which was different from the behaviors of salmon DNA. The flexible oligo-ssDNA formed rich structures that were subject to environmental disturbance. Kinetic study also indicated that the structures of the complex formed by oligo-ssDNA took much longer to mature than the structures formed by oligo-dsDNA. We attributed this result to the conformational adjustment of oligo-ssDNA in the complex.; Chemistry, Multidisciplinary; SCI(E); EI; 0; ARTICLE; dliang@pku.edu.cn; 12; 1738-1745; 57
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150368]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Niu Lin,Yan JingJing,Yang XuYan,et al. Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering[J]. science china chemistry,2014.
APA	Niu Lin.,Yan JingJing.,Yang XuYan.,Burger, Christian.,Rong LiXia.,...&Liang DeHai.(2014).Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering.science china chemistry.
MLA	Niu Lin,et al."Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering".science china chemistry (2014).