Preheating induced homogeneity of the small heat shock protein from   Methanococcus jannaschii

doi:10.1016/j.bbapap.2007.12.008

CORC > 北京大学 > 化学与分子工程学院

	Preheating induced homogeneity of the small heat shock protein from Methanococcus jannaschii
	Cao, Aoneng ; Wang, Zheng ; Wei, Ping ; Xu, Fei ; Cao, Jie ; Lai, Luhua
刊名	biochimica et biophysica acta proteins and proteomics
	2008
关键词	heat shock protein chaperone protein folding homogeneity HSP16.5 CHAPERONE-LIKE ACTIVITY SUBUNIT EXCHANGE ALPHA-CRYSTALLIN MYCOBACTERIUM-TUBERCULOSIS ACTIVATION MECHANISM UNFOLDING PROTEINS BOUNDARY ANALYSIS DISSOCIATION TEMPERATURE OLIGOMER
DOI	10.1016/j.bbapap.2007.12.008
英文摘要	Small heat shock proteins usually exhibit increased chaperone-like activity either at high temperatures or after preheating. However, the activation mechanism is still unclear. In the current study, we investigated the preheating-activation process of Mj HSP16.5, using various biophysical methods. Although Mj HSP16.5 was reported to be the most monodispersed sHSPs, we found that the newly purified Mj HSP16.5 was actually heterogeneous. 85 degrees C-preheating could activate Mj HSP16.5 and turn it into a more compact homogeneous species at the same time. Different cooling rates after preheating did not change the activity of Mj HSP16.5, suggesting that the 85 degrees C-preheated Mj HSP16.5 is in the most active and also the most stable state. These results demonstrate that the activation process of Mj HSP16.5 might accompany a refolding process. (C) 2007 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000254780200007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 8; ARTICLE; 3; 489-495; 1784
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150253]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cao, Aoneng,Wang, Zheng,Wei, Ping,et al. Preheating induced homogeneity of the small heat shock protein from Methanococcus jannaschii[J]. biochimica et biophysica acta proteins and proteomics,2008.
APA	Cao, Aoneng,Wang, Zheng,Wei, Ping,Xu, Fei,Cao, Jie,&Lai, Luhua.(2008).Preheating induced homogeneity of the small heat shock protein from Methanococcus jannaschii.biochimica et biophysica acta proteins and proteomics.
MLA	Cao, Aoneng,et al."Preheating induced homogeneity of the small heat shock protein from Methanococcus jannaschii".biochimica et biophysica acta proteins and proteomics (2008).