A CE-LIF method to monitor autophagy by directly detecting LC3 proteins   in HeLa cells

doi:10.1039/c2an36011j

CORC > 北京大学 > 化学与分子工程学院

	A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells
	Jin, Yue ; Chen, Cheng ; Meng, Lingchen ; Chen, Jitao ; Li, Meixian ; Zhu, Zhiwei ; Lin, Jian
刊名	analyst
	2012
关键词	INDUCED FLUORESCENCE DETECTION CAPILLARY-ELECTROPHORESIS MAMMALIAN AUTOPHAGY MARKER
DOI	10.1039/c2an36011j
英文摘要	A fast, simple and quantitative approach was established for monitoring autophagy in HeLa cells by directly detecting the conversion of green fluorescent protein (GFP) labelled autophagy markers, GFP-LC3-I to GFP-LC3-II, in crude cellular extract using capillary electrophoresis (CE) with laser-induced fluorescence (LIF). Compared with the traditional methods, this proposed method is simpler and more reliable. Moreover, high sensitivity, with a limit of detection (LOD) of 0.48 ppt (bovine serum albumin as standard protein), was obtained by an in-capillary derivatization method coupled with a field-enhanced sample injection (FESI) technique, this may allow the success of this technique in the detection of endogenous markers of autophagy in cells.; Chemistry, Analytical; SCI(E); PubMed; 2; ARTICLE; 23; 5571-5575; 137
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149880]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Jin, Yue,Chen, Cheng,Meng, Lingchen,et al. A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells[J]. analyst,2012.
APA	Jin, Yue.,Chen, Cheng.,Meng, Lingchen.,Chen, Jitao.,Li, Meixian.,...&Lin, Jian.(2012).A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells.analyst.
MLA	Jin, Yue,et al."A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells".analyst (2012).