Continuous monitoring of bisulfide variation in microdialysis effluents   by on-line droplet-based microfluidic fluorescent sensor

doi:10.1016/j.bios.2013.12.056

CORC > 北京大学 > 化学与分子工程学院

	Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor
	Zhu, Xiaocui ; Xu, Lei ; Wu, Tongbo ; Xu, Anqin ; Zhao, Meiping ; Liu, Shaorong
刊名	生物传感器与生物电子学
	2014
关键词	Hydrogen sulfide Gold nanoparticle Droplet-based microfluidics Microdialysis effluents On-line detection GAS-LIQUID FLOW HYDROGEN-SULFIDE IN-VIVO RAPID DETECTION PROBES MOLECULE TISSUES BLOOD
DOI	10.1016/j.bios.2013.12.056
英文摘要	We demonstrate a novel fluorescent sensor for real-time and continuous monitoring of the variation of bisulfide in microdialysis effluents by using a nanoparticle-glutathione-fluorescein isothiocyanate (AuNP-GSH-FITC) probe coupled with on-line droplet-based microfluidic chip. The AuNP-GSH-FITC fluorescent probe was firstly developed and used for bisulfide detection in bulk solution by quantitative real-time PCR, which achieved a linear working range from 0.1 mu M to 5.0 mu M and a limit of detection of similar to 50 nM. The response time was less than 2 min. With the aid of co-immobilized thiol-polyethylene glycol, the probe exhibited excellent stability and reproducibility in high salinity solutions, including artificial cerebrospinal fluids (aCSF). By adding 0.1% glyoxal to the probe solution, the assay allowed quantification of bisulfide in the presence of cysteine at the micro-molarity level. Using the AuNP-GSH-FITC probe, a droplet-based microfluidic fluorescent sensor was further constructed for online monitoring of bisulfide variation in the effluent of microdialysis. By using fluorescence microscope-charge-coupled device camera as the detector, the integrated microdialysis/microfluidic chip device achieved a detection limit of 2.0 mu M and a linear response from 5.0 p.M to 50 mu M for bisulfide in the tested sample. The method was successfully applied for the on-line measurement of bisulfide variation in aCSF and serum samples. It will be a very useful tool for tracking the variation of bisulfide or hydrogen sulfide in extracellular fluids. (C) 2013 Elsevier B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); EI; PubMed; 4; ARTICLE; mpzhao@pku.edu.cn; shaorong.liu@ou.edu; 438-445; 55
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149769]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhu, Xiaocui,Xu, Lei,Wu, Tongbo,et al. Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor[J]. 生物传感器与生物电子学,2014.
APA	Zhu, Xiaocui,Xu, Lei,Wu, Tongbo,Xu, Anqin,Zhao, Meiping,&Liu, Shaorong.(2014).Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor.生物传感器与生物电子学.
MLA	Zhu, Xiaocui,et al."Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor".生物传感器与生物电子学 (2014).