Direct Detection of DNA below ppb Level Based on Thionin-Functionalized   Layered MoS2 Electrochemical Sensors

doi:10.1021/ac5027786

CORC > 北京大学 > 化学与分子工程学院

	Direct Detection of DNA below ppb Level Based on Thionin-Functionalized Layered MoS2 Electrochemical Sensors
	Wang, Tanyuan ; Zhu, Ruizhi ; Zhuo, Junqiao ; Zhu, Zhiwei ; Shao, Yuanhua ; Li, Meixian
刊名	analytical chemistry
	2014
关键词	HYDROGEN-EVOLUTION HORSERADISH-PEROXIDASE OXYGEN-REDUCTION NUCLEIC-ACIDS BIOSENSORS NANOPARTICLES ELECTROCATALYSIS AMPLIFICATION HYBRIDIZATION ELECTRODES
DOI	10.1021/ac5027786
英文摘要	A layered MoS2-thionin composite was prepared by sonicating their mixture in an ionic liquid and gradient centrifugation. Because DNA is rarely present in single-stranded form, either naturally or after PCR amplification, the composite was used for fabrication of a double-stranded DNA (dsDNA) electrochemical biosensor due to stable electrochemical response, intercalation, and electrostatic interaction of thionin with DNA. The linear range over dsDNA concentration from 0.09 ng mL(-1) to 1.9 ng mL(-1) is obtained, and moreover, it is suitable for the detection of single-stranded DNA (ssDNA). The biosensor has been applied to the detection of circulating DNA from healthy human serum, and satisfactory results have been obtained. The constructed DNA electrochemical biosensor shows potential application in the fields of bioanalysis and clinic diagnosis. Furthermore, this work proposes a new method to construct electrochemical biosensors based on MoS2 sheets.; Chemistry, Analytical; SCI(E); EI; PubMed; 5; ARTICLE; lmwx@pku.edu.cn; 24; 12064-12069; 86
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149650]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Tanyuan,Zhu, Ruizhi,Zhuo, Junqiao,et al. Direct Detection of DNA below ppb Level Based on Thionin-Functionalized Layered MoS2 Electrochemical Sensors[J]. analytical chemistry,2014.
APA	Wang, Tanyuan,Zhu, Ruizhi,Zhuo, Junqiao,Zhu, Zhiwei,Shao, Yuanhua,&Li, Meixian.(2014).Direct Detection of DNA below ppb Level Based on Thionin-Functionalized Layered MoS2 Electrochemical Sensors.analytical chemistry.
MLA	Wang, Tanyuan,et al."Direct Detection of DNA below ppb Level Based on Thionin-Functionalized Layered MoS2 Electrochemical Sensors".analytical chemistry (2014).