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	环丙沙星的光谱性质、质子化作用与荧光量子产率; Spectral properties, protonation and fluorescence quantum yield of ciprofloxacin
	刘翠格 ; 徐怡庄 ; 魏永巨 ; 赵晶 ; 齐剑 ; 王新红 ; 许振华 ; 吴瑾光
	2005
关键词	环丙沙星 荧光光谱 紫外吸收光谱 质子化作用 荧光量子产率 ciprofloxacin fluorescence spectrum ultraviolet absorption spectrum protonation fluorescence quantum yield
英文摘要	研究了环丙沙星(ciprofloxacin,CIP)在不同pH条件下的荧光光谱、紫外吸收光谱和质子化作用,测量了CIP在中性条件下的荧光量子产率。在H+浓度大于1mol·L-1的HCl介质中,CIP分子(简写为HL)可以结合3个质子而以H4L3+形式存在,有微弱的荧光,最大荧光发射波长(λmax)为456nm。在pH0～2的酸性条件下,CIP主要以H3L2+形式存在,λmax为450nm,荧光较弱,荧光强度随pH的升高而上升。在pH2～4时,CIP主要以H2L+形式存在,具有强荧光,λmax仍为450nm。当pH>4时,λmax逐步蓝移到414nm,荧光强度随pH的升高而稍有降低,同时紫外吸收...; Fluorescence spectra, ultraviolet absorption spectra, and protonation of Ciprofloxacin (CIP) at different pH values have been studied. Fluorescence quantum yield of CIP under neutral condition has been measured. In HCl medium with [H+] > 1 mol x L(-1), CIP molecules (simplified as HL) may accept three protons to exist as H4L3+ with very weak fluorescence, and its maximum fluorescence emission wavelength (lambdamax) is 456 nm. In acidic solution of pH 0 to pH 2, CIP mainly exists as H3L2+ form with lambdamax at 450 nm, and fluorescence intensity is relatively weak and increases with increasing of pH. In the range of pH 2 to pH 4, CIP mainly exists as H2L+ form with a strong fluorescence, and lambdamax is still at 450 nm. When pH>4, lambdamax gradually blue-shifts to 414 nm, fluorescence intensity slightly decreases as pH increases, and at the same time an evident change in ultraviolet absorption spectrum is observed, indicating that H2L+ has lost proton to exist as dipole ion form HL. When pH>8, the fluorescence intensity decreases until disappearance as pH increases, indicating that HL has lost proton to exist as non-fluorescence anion ion form L-. In the molecular form changing process, the maximum excitation wavelength of CIP is essentially constant at 275 nm, but the maximum emission wavelength changes obviously. In a buffer solution with pH 7.0, and using quinine bisulphate as a reference, the fluorescence quantum yield of CIP at maximum excitation wavelength 275 nm was measured to be 0.12.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000232296200019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); EI; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 11; 09; 1446-1450; 25
语种	中文
出处	PubMed ; SCI ; 万方 ; 知网
出版者	光谱学与光谱分析
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/77928]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	刘翠格,徐怡庄,魏永巨,等. 环丙沙星的光谱性质、质子化作用与荧光量子产率, Spectral properties, protonation and fluorescence quantum yield of ciprofloxacin. 2005-01-01.

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