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毛细管电泳免疫分析法分析雌三醇; Determination of Estriol by Capillary Electrophoretic Immunoassay
苏萍 ; 王永成 ; 张新祥 ; 常文保
2003
关键词毛细管电泳 免疫分析 雌三醇 激光诱导荧光检测器 水凝胶 capillary electrophoresis immunoassay estriol laser-induced fluorescence detection hydrogel
英文摘要利用一种温敏水凝胶作为毛细管电泳的填充介质,建立了毛细管电泳免疫分析血清中雌三醇的方法.研究了缓冲溶液的浓度和pH值、水凝胶的浓度、电压等因素对分析结果的影响.雌三醇的检出限和线性范围分别为31.6 ng/L和50~5000 ng/L.; A kind of thermally linear polymer-poly-N-isopropylacrylamide (PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estroil antigen. A useful method for the determination of estroil in serum by capillary electrophoretic immunoassay was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage etc. has been studied. The limit of detection and the linear range of estriol were 31.6 ng/L and 50 similar to 5000 ng/L, respectively.; 国家自然科学基金; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000182746400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 3; 4; 385-388; 31
语种中文
出处SCI ; 万方 ; http://d.g.wanfangdata.com.cn/Periodical_fxhx200304002.aspx
出版者分析化学
内容类型其他
源URL[http://hdl.handle.net/20.500.11897/46988]  
专题化学与分子工程学院
推荐引用方式
GB/T 7714
苏萍,王永成,张新祥,等. 毛细管电泳免疫分析法分析雌三醇, Determination of Estriol by Capillary Electrophoretic Immunoassay. 2003-01-01.
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