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	检测痕量Hg2+的DNA电化学生物传感器; DNA Electrochemical Biosensor for Trace Hg2+ Detection
	戈芳 ; 曹瑞国 ; 朱斌 ; 李经建 ; 徐东升
	2010
关键词	生物传感器 构象变化 示差脉冲伏安法 电化学阻抗谱 Hg2+ DNA Biosensor Hg2+ DNA Conformational change Differential pulse voltammetry Electrochemical impedance spectroscopy
英文摘要	通过自组装方法将修饰有二茂铁基团的富T序列DNA分子(DNA-Fc)固定在金电极表面,得到了一种基于DNA修饰电极的电化学汞离子(Hg2+)传感器.当溶液中有Hg2+存在时,Hg2+可与修饰电极上DNA的T碱基发生较强的特异结合,形成T-Hg2+-T发卡结构,使DNA分子构象发生改变,其末端具有电化学活性的二茂铁基团远离电极表面,电化学响应随之发生变化.示差脉冲伏安法(DPV)结果显示:DNA末端二茂铁基团的还原峰在0.26 V(vs饱和甘汞电极(SCE))附近,峰电流随溶液中Hg2+浓度的增加而降低;Hg2+浓度范围在0.1 nmol·L-1-1 μmol·L-1时,电流相对变化率与Hg2+浓度的对数呈现良好的线性关系.该修饰电极对Hg2+的检测限为0.1nmol·L-1,可作为痕量Hg2+检测的电化学生物传感器.干扰实验也表明,该传感器对Hg2+具有良好的特异性与灵敏度.; In this paper we demonstrated a novel type of electrochemical Hg2+ biosensor based on a DNA-modified electrode. Ferrocenyl. modified T. rich DNA (DNA-Fc) molecules were synthesized for use as electrochemical probes. We then fixed these DNA-Fc probes onto a gold electrode surface by self. assembly. In the presence of Hg2+, the single strand DNA on the electrode surface turned to a thymine-Hg2+-thymine (T-Hg2+-T) hairpin structure. The ferrocenyl groups were kept away from the surface of the electrode, and this could be measured sensitively by differential pulse voltammetry (DPV). The results show a reduction peak of ferrocene at 0.26 V (vs saturated calomel electrode (SCE)) and the peak current of DPV decreased with increasing the concentration of Hg2+. The rate of current change is linear with regards to lgc(Hg2+) over a concentration range from 0.1 nmol . L-1 to 1 mu mol . L-1 and with a detection limit of 0.1 mol . L-1. A test for interference metal ions showed that this electrochemical biosensor based on a DNA modified electrode is highly sensitive and selective, and it can be widely used for trace Hg2+ detection.; 国家自然科学基金; 国家重点基础研究项目特别基金; 国家重点基础研究发展计划项目973); http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000279776400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 5; 7; 1779-1783; 26
语种	中文
出处	万方 ; SCI ; http://d.g.wanfangdata.com.cn/Periodical_wlhxxb201007008.aspx
出版者	物理化学学报
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/46789]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	戈芳,曹瑞国,朱斌,等. 检测痕量Hg2+的DNA电化学生物传感器, DNA Electrochemical Biosensor for Trace Hg2+ Detection. 2010-01-01.

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