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	The exon junction complex regulates the splicing of cell polarity gene dig1 to control Wingless signaling in development
	Liu, Min ; Li, Yajuan ; Liu, Aiguo ; Li, Ruifeng ; Su, Ying ; Du, Juan ; Li, Cheng ; Zhu, Alan Jian
	2016
关键词	D. melanogaster,Dishevelled,RNA splicing,Wnt/Wingless signaling,cell biology,developmental biology,discs large,exon junction complex,stem cells OSKAR MESSENGER-RNA BINDING PROTEIN POSTERIOR LOCALIZATION FRIZZLED RECEPTORS MAGO NASHI DROSOPHILA PATHWAY WNT DEGRADATION UBIQUITINATION
英文摘要	Wingless (Wg)/Wnt signaling is conserved in all metazoan animals and plays critical roles in development. The Wg/Wnt morphogen reception is essential for signal activation, whose activity is mediated through the receptor complex and a scaffold protein Dishevelled (Dsh). We report here that the exon junction complex (EJC) activity is indispensable for Wg signaling by maintaining an appropriate level of Dsh protein for Wg ligand reception in Drosophila. Transcriptome analyses in Drosophila wing imaginal discs indicate that the EJC controls the splicing of the cell polarity gene discs large 1 (dlg1), whose coding protein directly interacts with Dsh. Genetic and biochemical experiments demonstrate that DIg1 protein acts independently from its role in cell polarity to protect Dsh protein from lysosomal degradation. More importantly, human orthologous Dig protein is sufficient to promote Dv1 protein stabilization and Wnt signaling activity, thus revealing a conserved regulatory mechanism of Wg/Wnt signaling by Dig and EJC.; State Key Laboratory of Membrane Biology of the People's Republic of China; Peking-Tsinghua Center for Life Sciences; Ministry of Science and Technology of the People's Republic of China [2014CB942804]; National Science Foundation of the People's Republic of China [31371410, 31401241]; China Postdoctoral Science Foundation [2014M550556]; SCI(E); PubMed; ARTICLE; zhua@pku.edu.cn; 5
语种	英语
出处	PubMed ; SCI
出版者	ELIFE
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/493217]
专题	数学科学学院 生命科学学院
推荐引用方式 GB/T 7714	Liu, Min,Li, Yajuan,Liu, Aiguo,et al. The exon junction complex regulates the splicing of cell polarity gene dig1 to control Wingless signaling in development. 2016-01-01.

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