Characterization and Comparative Analysis of Wheat High Molecular Weight Glutenin Subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS

	Characterization and Comparative Analysis of Wheat High Molecular Weight Glutenin Subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS
	Gao, Liyan ; Ma, Wujun ; Chen, Jing ; Wang, Ke ; Li, Jing ; Wang, Shunli ; Bekes, Frank ; Appels, Rudi ; Yan, Yueming
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2010
卷号	58 期号:5 页码:2777-2786
关键词	HMW-GS RP-HPLC HPCE MALDI-TOF-MS wheat
ISSN号	0021-8561
通讯作者	Yan, YM, Capital Normal Univ, Key Lab Genet & Biotechnol, Coll Life Sci, Xisanhuan Beilu 105, Beijing 100048, Peoples R China
中文摘要	High molecular weight glutenin subunits (HMW-GS) from 60 germplasms including 30 common wheat cultivars and 30 related species were separated and characterized by a suite of separation methods including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), reversed-phase high-performance liquid chromatography (RP-HPLC), high-performance capillary electrophoresis (HPCE), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Comparative analysis demonstrated that each methodology has its own advantages and disadvantages, The main drawback of SIDS-PAGE was its overestimation of molecular mass and incorrect identification of HMW-GS due to its low resolution. However, it had the advantages of technical simplicity and low requirements of equipment; thus, it is suitable for large-scale and high-throughput HMW-GS screening for breeding programs, especially when the glutenin composition is clear in the breeding material. MALDI-TOF-MS clearly expressed many technical advantages among the four methods evaluated, including high throughput, high resolution, and accuracy; it was, however, associated with high equipment cost, thus preventing many breeding companies from accessing the technology. RP-HPLC and HPCE were found to be intermediate between SIDS-PAGE and MALDI-TOF-MS. Both RP-HPLC and HPCE demonstrated higher resolution and reproducibility over SDS-PAGE but lower detection power than MALDI-TOF-MS. Results demonstrated that MALDI-TOF-MS is suitable for analyzing HMW-GS for routine breeding line screening and for identifying new genotypes.
学科主题	Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
收录类别	SCI
资助信息	National Natural Science Foundation of China [30830072, 30871527, 30771334]; Chinese Ministry of Science and Technology [2009CB118303, 12006-AA10Z186]; National Key Project for Transgenic Crops [2009ZX08009-010B, 2009ZX08002-003B]; Beijing Municipal Commission of Education [KZ200910028003]
语种	英语
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/22532]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Gao, Liyan,Ma, Wujun,Chen, Jing,et al. Characterization and Comparative Analysis of Wheat High Molecular Weight Glutenin Subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2010,58(5):2777-2786.
APA	Gao, Liyan.,Ma, Wujun.,Chen, Jing.,Wang, Ke.,Li, Jing.,...&Yan, Yueming.(2010).Characterization and Comparative Analysis of Wheat High Molecular Weight Glutenin Subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,58(5),2777-2786.
MLA	Gao, Liyan,et al."Characterization and Comparative Analysis of Wheat High Molecular Weight Glutenin Subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 58.5(2010):2777-2786.