Nanoparticles with High-Surface Negative-Charge Density Disturb the Metabolism of Low-Density Lipoprotein in Cells

doi:10.3390/ijms19092790

	Nanoparticles with High-Surface Negative-Charge Density Disturb the Metabolism of Low-Density Lipoprotein in Cells
	Bai X(白雪); Zhang JX(张佳欣); Chang YN(常亚男); Gu WH(古伟宏); Lei RH(雷润宏); Qin YX(秦艳霞); Xia SB(夏士博); Ma SH(马思汉); Liang YL(梁月兰); Chen K(陈奎)
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2018
卷号	19 期号:9 页码:2790
关键词	low-density lipoprotein endocytosis gold particles surface charge density
ISSN号	1422-0067
DOI	10.3390/ijms19092790
文献子类	Article
英文摘要	Endocytosis is an important pathway to regulate the metabolism of low-density lipoprotein (LDL) in cells. At the same time, engineering nanoparticles (ENPs) enter the cell through endocytosis in biomedical applications. Therefore, a crucial question is whether the nanoparticles involved in endocytosis could impact the natural metabolism of LDL in cells. In this study, we fabricated a series of gold nanoparticles (AuNPs) (13.00 +/- 0.69 nm) with varied surface charge densities. The internalized AuNPs with high-surface negative-charge densities (HSNCD) significantly reduced LDL uptake in HepG-2, HeLa, and SMMC-7721 cells compared with those cells in control group. Notably, the significant reduction of LDL uptake in cells correlates with the reduction of LDL receptors (LDL-R) on the cell surface, but there is no change in protein and mRNA of LDL-Rs. The cyclic utilization of LDL-R in cells is a crucial pathway to maintain the homoeostasis of LDL uptake. The release of LDL-Rs from LDL/LDL-R complexes in endosomes depended on reduction of the pH in the lumen. AuNPs with HSNCD hampered vacuolar-type H+-ATPase V1 (ATPaseV1) and ATPaseV0 binding on the endosome membrane, blocking protons to enter the endosome by the pump. Hence, fewer freed LDL-Rs were transported into recycling endosomes (REs) to be returned to cell surface for reuse, reducing the LDL uptake of cells by receptor-mediated endocytosis. The restrained LDL-Rs in the LDL/LDL-R complex were degraded in lysosomes.
WOS关键词	RECEPTOR-MEDIATED ENDOCYTOSIS ; FUNCTIONALIZED GOLD NANOPARTICLES ; HUMAN-FIBROBLASTS ; ACIDIFICATION ; CHOLESTEROL ; HOMEOSTASIS ; EXOCYTOSIS ; VESICLES ; DELIVERY ; GROWTH
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000449988100326
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286719]
专题	高能物理研究所_多学科研究中心高能物理研究所_实验物理中心高能物理研究所_加速器中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Bai X,Zhang JX,Chang YN,et al. Nanoparticles with High-Surface Negative-Charge Density Disturb the Metabolism of Low-Density Lipoprotein in Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(9):2790.
APA	白雪.,张佳欣.,常亚男.,古伟宏.,雷润宏.,...&Qin, Y..(2018).Nanoparticles with High-Surface Negative-Charge Density Disturb the Metabolism of Low-Density Lipoprotein in Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(9),2790.
MLA	白雪,et al."Nanoparticles with High-Surface Negative-Charge Density Disturb the Metabolism of Low-Density Lipoprotein in Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.9(2018):2790.