Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

	Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
	Zhou, Y ; Xu, G ; Choi, FFK ; Ding, LS ; Bin Han, Q ; Song, JZ ; Qiao, CF ; Zhao, QS ; Xu, HX
刊名	JOURNAL OF CHROMATOGRAPHY A
	2009
卷号	1216 期号:24 页码:4847_4858
关键词	Salvia species UHPLC-QTOF-MS/MS Diterpenoids Fragmentation pathway Quantification Principal component analysis
ISSN号	0021-9673
通讯作者	Zhao, QS, Chinese Acad Sci, Kunming Inst Bot, State Key LPhytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China.
产权排序	3
中文摘要	An on-line ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode-array detection and quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) method has been optimized and established for the qualitative and quantitative analysis of the important diterpenoids in the methanol extracts of 12 Salvia species. Specific marker components were identified for the classification of the Salvia samples by principal component analysis. The accurate mass measurement within 3 ppm error for all the protonated molecules and Subsequent fragment ions offers higher quality structural information for interpretation of fragmentation pathways of various groups of diterpenoids. Thus. a total of 21 diterpenoids from different Salvio species were separated within 10 min, and were unequivocally or tentatively identified via comparisons with authentic standards and literature. This UHPLC-QTOF-MS/MS method was validated to be sensitive, precise and accurate with the limit of detections at 3.0-16 ng/ml. and the overall intra-day and the inter-clay variations less than 3%. The recovery of the method was in the range of 96.2-101.8%, with relative standard deviation (RSD) less than 3.0%. The results demonstrated that the qualitative and quantitative differences in diterpenoids were not only useful for chemotaxonomy in some Salvia species but also for the standardization and differentiation of large numbers of similar samples. (C) 2009 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods; Chemistry, Analytical
收录类别	SCI
资助信息	Hong Kong Jockey Club Charities Trust Fund ; National Natural Science Foundation of China [20702054]; Natural Science Foundation of Yunnan Province [2006CO044Q]
语种	英语
WOS记录号	WOS:000266671400005
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18267]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhou, Y,Xu, G,Choi, FFK,et al. Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(24):4847_4858.
APA	Zhou, Y.,Xu, G.,Choi, FFK.,Ding, LS.,Bin Han, Q.,...&Xu, HX.(2009).Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1216(24),4847_4858.
MLA	Zhou, Y,et al."Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1216.24(2009):4847_4858.