Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography

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	Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography
	Liu, YH; Yin, H; Zhang, S; Luo, XM; zhs_1999@hotmail.com
刊名	JOURNAL OF CHROMATOGRAPHY A
	2008
卷号	1205 期号:40545 页码:177-181
关键词	Benzoxazinoids High-speed Counter-current Chromatography (Hsccc) Acanthus Ilicifolius l (2r)-2-o-beta-d-glucopyranosyl-2h-1 (2r)-2-o-beta-d-glucopyranosyl-4-hydroxy-2h-1 4-benzoxazin-3(4h)-one (Hboa-glc) 4-benzoxazin-3(4h)-one (Diboa-glc)
ISSN号	0021-9673
英文摘要	The first preparative separation of two benzoxazinoids, (2R)-2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one (HBOA-Glc) and (2R)-2-O-beta-D-glucopyranosyl-4-hydroxy-2H-1,4-benzoxazin3(4H)-one (DIBOA-Glc), by means of high-speed counter-current chromatography (HSCCC) from the n-butanol extract of Acanthus ilicifolius L. is presented. The two-phase solvent system containing ethyl acetate-n-butanol-0.5%NH4OH (2:3:5, v/v/v, system 13) was selected for the one-step HSCCC separation of HBOA-Glc and DIBOA-Glc according to the partition coefficient values (K) for target compounds and the separation factor (alpha) between the two target compounds. In the one-step HSCCC separation using solvent B, from 100 mg n-butanol extract of A. ilicifolius, 6.3 mg HBOA-Glc and 6.8 mg DIBOA-Glc were isolated with purities of 90.3% and 80.2%, respectively. In order to obtain the two target compounds with higher purity, a second separation process was developed comprising two steps. In the two-step separation, the sample was first pre-purified by HSCCC using ethyl acetate-n-butanol-water (2:3:5, v/v/v, system A) solvent system and then purified using solvent system B. A 100-mg amount of the n-butanol extracts of A. ilicifolius was separated to yield 5.8 mg of HBOA-Glc and 4.8 mg of DIBOA-Glc with purities of 97.1% and 94.8%, respectively, which were directly used for NMR analyses. (C) 2008 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods ; Chemistry, Analytical
URL标识	查看原文
资助项目	广东省海洋药物重点实验室
WOS记录号	WOS:000259884700022
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5262]
专题	南海海洋研究所_广东省海洋药物重点实验室
通讯作者	zhs_1999@hotmail.com
推荐引用方式 GB/T 7714	Liu, YH,Yin, H,Zhang, S,et al. Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1205(40545):177-181.
APA	Liu, YH,Yin, H,Zhang, S,Luo, XM,&zhs_1999@hotmail.com.(2008).Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1205(40545),177-181.
MLA	Liu, YH,et al."Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1205.40545(2008):177-181.