Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans
	Zhang, CS; Ma, JY; Zuo, DG; Song, YX; Wang, B; Huang, HB; Yao, YL; Li, WJ; Zhang, S; Ju, JH
刊名	CHEMBIOCHEM
	2012
卷号	13 期号:4 页码:547-552
关键词	Biosynthesis Methylpendolmycin Natural Products Pendolmycin Prenyltransferases
ISSN号	1439-4227
英文摘要	The nine-membered indolactam antibiotics belong to a small group of antibiotics showing broad biological activities. However, the in vivo genetic engineering of compounds of this type has not been performed. Here we report the identification of a single gene cluster responsible for the biosynthesis of methylpendolmycin and pendolmycin, two members of this family of antibiotics, from the deep sea bacterium Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis and gene inactivation, coupled with metabolite characterization, reveal that MpnB, a nonribosomal peptide synthetase, MpnC, a cytochrome P450, and MpnD, a prenyltransferase, are sufficient to catalyze the biosynthesis of the two antibiotics from L-Ile (or L-Val), L-Trp, and methionine. MpnD is the first identified enzyme that transfers a C5 prenyl unit in a reverse manner to the C-7 position of a Trp-derived natural product.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Medicinal
资助项目	LMB
语种	英语
WOS记录号	WOS:000300871600013
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9958]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	jju@scsio.ac.cn
推荐引用方式 GB/T 7714	Zhang, CS,Ma, JY,Zuo, DG,et al. Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans[J]. CHEMBIOCHEM,2012,13(4):547-552.
APA	Zhang, CS.,Ma, JY.,Zuo, DG.,Song, YX.,Wang, B.,...&jju@scsio.ac.cn.(2012).Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans.CHEMBIOCHEM,13(4),547-552.
MLA	Zhang, CS,et al."Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans".CHEMBIOCHEM 13.4(2012):547-552.