Actinomadura sediminis sp nov., a marine actinomycete isolated from mangrove sediment

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	Actinomadura sediminis sp nov., a marine actinomycete isolated from mangrove sediment
	Nie, GX; wjli@ynu.edu.cn; oceanactino@gmail.com; Li, WJ; Sivakumar, K; Zhang, S; Xie, Q; Tian, XP; Sahu, MK; Xu, Y
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2012
卷号	62 页码:1110-1116
ISSN号	1466-5026
英文摘要	In this study, the taxonomic position of an actinobacterium, strain YIM M 10931(T), which was isolated from a mangrove sediment sample collected in Dugong Creek, Little Andaman, India, was determined by a polyphasic approach. This Gram-positive, aerobic strain produced branched substrate mycelium and aerial hyphae, which differentiated into short, hooked or spiral spore chains. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole cell sugars consisted of mannose, ribose, glucose, galactose and madurose. The cellular fatty acid profile mainly consisted of iso-C-16:0, 10-methyl C-18:0 and C-16:0. The quinone system was predominantly composed of MK-9(H-8) (45.5%) and MK-9(H-6) (39%). The phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and two unknown phospholipids. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus Actinomadura. Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relatives as Actinomadura cremea DSM 43676(T) (98.4% sequence similarity), Actinomadura rifamycini DSM 43936(T) (97.4%) and Actinomadura apis IM17-1(T) (96.9%). It was obvious from the resulting phylogenetic trees that strain YIM M 10931(T) belongs to a distinct subclade within the evolutionary radiation of the genus Actinomadura. DNA-DNA hybridizations of strain YIM M 10931(T) with A. cremea DSM 43676(T) and A. rifamycini DSM 43936(T) were performed and further confirmed that the isolate represents a separate genomic species. Based on the phenotypic and genotypic characteristics presented, it is proposed that strain YIM M 10931(T) represents a novel species within the genus Actinomadura, for which the name Actinomadura sediminis sp. nov. is proposed; the type strain is YIM M 10931(T) (= CCTCC AA 2010009(T)= DSM 45500(T)).
学科主题	Microbiology
资助项目	LMB
语种	英语
WOS记录号	WOS:000305367800016
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9906]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	wjli@ynu.edu.cn; oceanactino@gmail.com
推荐引用方式 GB/T 7714	Nie, GX,wjli@ynu.edu.cn,oceanactino@gmail.com,et al. Actinomadura sediminis sp nov., a marine actinomycete isolated from mangrove sediment[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2012,62:1110-1116.
APA	Nie, GX.,wjli@ynu.edu.cn.,oceanactino@gmail.com.,Li, WJ.,Sivakumar, K.,...&He, J.(2012).Actinomadura sediminis sp nov., a marine actinomycete isolated from mangrove sediment.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,62,1110-1116.
MLA	Nie, GX,et al."Actinomadura sediminis sp nov., a marine actinomycete isolated from mangrove sediment".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62(2012):1110-1116.