Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting

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	Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting
	Wang, YS; Zhang, YY; Dong, JD; amy0517@gmail.com; Yang, B; Ling, J; Zhang, S
刊名	SCIENTIA MARINA
	2009
卷号	73 期号:3 页码:487-498
关键词	Bacterial Community Mangrove Sediments Denaturing Gradient Gel Electrophoresis Multivariate Statistics
ISSN号	0214-8358
英文摘要	Bacterial community structure and the relationship between environmental variables and microbial communities in the surface sediments of tropical mangrove ecosystems were investigated in Sanya, Hainan Island, China. Profiles of bacterial communities were generated using culture-independent PCR-denaturing gradient get electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis. Findings suggested that microbial communities varied with sample collection sites and seasons. The samples collected from the same sample sites tit the same time had more similar microbial communities except samples SH3 and AB5, which also had unique sediment quality. Canonical correspondence analysis (CCA) revealed that the organic carbon concentration of the sediments accounted for a significant amount of the variability in the bacterial community composition. Phylogenetic analysis was used to identify the major groups of the predominant bacterial phylotypes. 16S rRNA gene-V3 fragments from 17 individual DGGE bands were sequenced and the corresponding bacteria were found in mangrove sediments for the first time based on BLAST results. Predominant bacterial phylotypes Clustered with various taxonomic groups, including Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria and Firmicutes.
学科主题	Marine & Freshwater Biology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000269965600007
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5068]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	amy0517@gmail.com
推荐引用方式 GB/T 7714	Wang, YS,Zhang, YY,Dong, JD,et al. Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting[J]. SCIENTIA MARINA,2009,73(3):487-498.
APA	Wang, YS.,Zhang, YY.,Dong, JD.,amy0517@gmail.com.,Yang, B.,...&Zhang, S.(2009).Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting.SCIENTIA MARINA,73(3),487-498.
MLA	Wang, YS,et al."Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting".SCIENTIA MARINA 73.3(2009):487-498.