Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization

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	Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization
	Shi, Y; Zhao, Z; Yin, JK; Zhu, XP; Chen, KC; Pan, DB; Gui, JF; zhuxinping_1964@yahoo.com.cn
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2010
卷号	155 期号:2 页码:110-117
关键词	Interferon Regulator Factor-2 (Irf-2) Epinephelus Coioides Genomic Structure Inductive Expression Subcellular Localization
ISSN号	1096-4959
英文摘要	Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper. (C) 2009 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Zoology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000273927000003
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3912]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	zhuxinping_1964@yahoo.com.cn
推荐引用方式 GB/T 7714	Shi, Y,Zhao, Z,Yin, JK,et al. Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,155(2):110-117.
APA	Shi, Y.,Zhao, Z.,Yin, JK.,Zhu, XP.,Chen, KC.,...&zhuxinping_1964@yahoo.com.cn.(2010).Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,155(2),110-117.
MLA	Shi, Y,et al."Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 155.2(2010):110-117.