Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi
	Lu, YS; Cai, SH; wuzh@gdou.edu.cn; Jian, JC; Li, Y; Pang, HY; Wu, ZH
刊名	JOURNAL OF APPLIED MICROBIOLOGY
	2010
卷号	109 期号:5 页码:1800-1809
关键词	Immunoproteome Vibrio Harveyi Whole-cell Protein
ISSN号	1364-5072
英文摘要	Aims: The main aim of this study was to screen novel immunogenic proteins of Vibrio harveyi, which could be vaccine candidates. Methods and Results: Whole-cell proteins of V. harveyi, strain Li01 and Huang01, were first separated by isoelectric focusing, followed by 2D-PAGE, respectively. Immunogenic proteins were identified by Western blotting, using Epinephelus coioides antisera against V. harveyi strain Li01. Western blot analyses revealed 16 shared immunogenic protein spots in both strains. All of the immunogenic proteins were successfully identified and corresponded to 15 proteins. None of these proteins have been previously reported as immunogenic for V. harveyi. Of the 15 proteins, 11 are specific immunoreactive proteins and four are nonspecific immunoreactive proteins. Furthermore, outer membrane protein N (spot 2) and oligopeptide ATP-binding cassette (ABC) transporter (spot 3) were used as immunogens to immunize E. coioides for investigation of their protective abilities and activities. The E. coioides immunized with OmpN has abilities to fight against infections caused by V. harveyi Li01 and Huang01. However, vaccination with oligopeptide ABC transporter induces low protective immune response in fish. Conclusions: Eleven novel specific antigens were found, and OmpN could potentially be used as vaccine candidate for the development of novel vaccine against V. harveyi. Significance and Impact of the Study: These data show that immunoproteomics methods can be successfully applied in identifying immunogenic proteins of V. harveyi, which helps to search for the protective antigens in future.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000282813800031
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3750]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	wuzh@gdou.edu.cn
推荐引用方式 GB/T 7714	Lu, YS,Cai, SH,wuzh@gdou.edu.cn,et al. Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi[J]. JOURNAL OF APPLIED MICROBIOLOGY,2010,109(5):1800-1809.
APA	Lu, YS.,Cai, SH.,wuzh@gdou.edu.cn.,Jian, JC.,Li, Y.,...&Wu, ZH.(2010).Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi.JOURNAL OF APPLIED MICROBIOLOGY,109(5),1800-1809.
MLA	Lu, YS,et al."Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi".JOURNAL OF APPLIED MICROBIOLOGY 109.5(2010):1800-1809.