Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative

doi:10.1007/s13205-019-1611-1

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	Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative
	Yang, Song ; Mo, Xuhua ; Gui, Chun 2
刊名	3 BIOTECH
	2019
卷号	9 期号:3 页码:71
关键词	Tirandamycins Streptolydigin Cytochrome P450 oxidase SlgO1 Combinatorial biosynthesis
ISSN号	2190-572X
DOI	10.1007/s13205-019-1611-1
英文摘要	In the present study, an Escherichia coli whole cell system with overexpression of a cytochrome P450 oxidase SlgO1 involved in streptolydigin biosynthetic pathway, an E. coli flavodoxin NADP(+) oxidoreductase (EcFLDR), and an E. coli flavodoxin A (EcFLDA) were constructed. Biotransformation experiments revealed that SlgO1 can convert tirandamycin C to tirandamycin F, indicating that it can introduce a hydroxyl group into the C-10 position of tirandamycin C. Subsequently, slgO1 was cloned into pSET152AKE vector under the downstream of ermE* promoter, which was, respectively, introduced into Streptomyces sp. SCSIO1666 (tirandamycin B producer), Streptomyces sp. Ju1008 (tirandamycin C producer), and Streptomyces sp. Ju1009 (tirandamycin E producer). A novel tirandamycin derivative tirandamycin L accumulated in the engineered strain Streptomyces sp. Ju1008::slgO1 was isolated and its structure was determined on the basis of nuclear magnetic resonance (NMR) and mass spectrometry. Unlike most of the identified tirandamycins, tirandamycin L possessed a rare C-11-C-12 saturated bond as well as a C-10 ketone moiety. In addition, tirandamycin L showed weaker antibacterial activity. Based on the structure of tirandamycin L, SlgO1 was proposed to be responsible for multiple modifications toward tirandamycin C, including the formation of C-10 hydroxyl and C-11-C-12 saturated bond.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17889]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 2.Qingdao Agr Univ, Shandong Prov Key Lab Appl Mycol, Sch Life Sci, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Yang, Song,Mo, Xuhua,Gui, Chun. Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative[J]. 3 BIOTECH,2019,9(3):71.
APA	Yang, Song,Mo, Xuhua,&Gui, Chun.(2019).Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative.3 BIOTECH,9(3),71.
MLA	Yang, Song,et al."Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative".3 BIOTECH 9.3(2019):71.