Transcriptome analysis of mantle tissues reveals potential biomineralization-related genes in Tectus pyramis Born

doi:10.1016/j.cbd.2018.11.010

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Transcriptome analysis of mantle tissues reveals potential biomineralization-related genes in Tectus pyramis Born
	Shi, Yu; Xu, Meng; Huang, Jing; Zhang, Hua; Liu, Wenguang; Ou, Zekui; He, Maoxian
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2019
卷号	29 页码:131
关键词	Tectus pyramis Transcriptome Biomineralization Mantle tissue qPCR Phylogenetic analysis
ISSN号	1744-117X
DOI	10.1016/j.cbd.2018.11.010
英文摘要	The marine mollusk Tectus pyramis is a valuable shellfish primarily distributed in the tropical waters of the South China Sea, as well as in the Indo-Pacific Ocean and areas near the southern portion of the Japanese Peninsula. Despite major economic interest in this mollusk, limited genomic resources are available for this species, which has prevented studies of the molecular mechanism, such as biomineralization. Here, we report the first comprehensive transcript dataset of T. pyramis mantle tissue. From a total of 16,801,141 reads, 173,671 unique transcripts were assembled, which provides new genomic resources for the understanding of biomineralization in T. pyramis. The most abundant unique sequences of the top 30 most highly expressed genes were annotated as shematrin, while other highly expressed genes included glycine-rich protein and shematrin-1. Based on transcriptome annotation and Gene Ontology classification, 130 biomineralization-related genes were found including members of the BMP (bone morphogenetic proteins), calmodulin, perlucin, and shematrin families, as well as mantle genes, nacrein, and MSI60. The results of qPCR showed that 14 of 24 examined genes were highly expressed in the mantle. A phylogenetic tree of BMP, perlucin, shematrin proteins revealed conservation of their structure and functions and indicated that some members participated in biomineralization in T. pyramis. Taken together, the results presented herein will be useful in studies of molecular mechanisms and pathways of biomineralization in T. pyramis, as well as provide new insight into the mechanisms of biomineralization in gastropods.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17885]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shi, Yu,Xu, Meng,Huang, Jing,et al. Transcriptome analysis of mantle tissues reveals potential biomineralization-related genes in Tectus pyramis Born[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2019,29:131, 144.
APA	Shi, Yu.,Xu, Meng.,Huang, Jing.,Zhang, Hua.,Liu, Wenguang.,...&He, Maoxian.(2019).Transcriptome analysis of mantle tissues reveals potential biomineralization-related genes in Tectus pyramis Born.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,29,131.
MLA	Shi, Yu,et al."Transcriptome analysis of mantle tissues reveals potential biomineralization-related genes in Tectus pyramis Born".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 29(2019):131.