Structure and allosteric coupling of type II antitoxin CopA(SO)

doi:10.1016/j.bbrc.2019.05.049

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	Structure and allosteric coupling of type II antitoxin CopA(SO)
	Zhang, Jiahai 5; Zhao, Rongjuan 1,2; Li, Quan 1,2; Zhang, Jingiing 1,2; Li, Fudong 5; Yao, Jianyun 3; Liu, Lin 1,2; Wang, Xiaoxue 3; Zhang, Xuecheng 1,2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2019
卷号	514 期号:4 页码:1122
关键词	Allosteric coupling Antitoxin CopA(SO) Ribbon-helix-helix motif Shewanella oneidensis
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2019.05.049
英文摘要	Toxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopAso, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopAso is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate. (C) 2019 Elsevier Inc. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17810]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Anhui Prov Engn Technol Res Ctr Microorganisms &, Hefei 230601, Anhui, Peoples R China 2.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn, RNAM Ctr Marine Microbio,South China Sea Inst Oce, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China 5.Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiahai,Zhao, Rongjuan,Li, Quan,et al. Structure and allosteric coupling of type II antitoxin CopA(SO)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,514(4):1122, 1127.
APA	Zhang, Jiahai.,Zhao, Rongjuan.,Li, Quan.,Zhang, Jingiing.,Li, Fudong.,...&Zhang, Xuecheng.(2019).Structure and allosteric coupling of type II antitoxin CopA(SO).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,514(4),1122.
MLA	Zhang, Jiahai,et al."Structure and allosteric coupling of type II antitoxin CopA(SO)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 514.4(2019):1122.