QQ客服 官方微博 反馈留言

	Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli
	Zeng, Jun2,3,4; Lin, Xiangui1,2,3; Zhang, Jing2,3; Li, Xuanzhen2,3,4; Wong, Ming Hung3,5
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2011-03-01
卷号	89期号:6页码:1841-1849
关键词	Anthracene Bacterial laccase Benzo[a]pyrene Biotransformation CueO Polycyclic aromatic hydrocarbons (PAHs)
ISSN号	0175-7598
DOI	10.1007/s00253-010-3009-1
通讯作者	Lin, Xiangui(xglin@issas.ac.cn)
英文摘要	Laccases produced by white rot fungi are capable of rapidly oxidizing benzo[a]pyrene. We hypothesize that the polycyclic aromatic hydrocarbon (PAH)-degrading bacteria producing laccase can enhance the degree of benzo[a]pyrene mineralization. However, fungal laccases are glycoproteins which cannot be glycosylated in bacteria, and there is no evidence to show that bacterial laccases can oxidize benzo[a]pyrene. In this study, the in vitro oxidation of PAHs by crude preparations of the bacterial laccase, CueO, from Escherichia coli was investigated. The results revealed that the crude CueO catalyzed the oxidation of anthracene and benzo[a]pyrene in the same way as the fungal laccase from Trametes versicolor, but showed specific characteristics such as thermostability and copper dependence. In the presence of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), high amounts of anthracene and benzo[a]pyrene, 80% and 97%, respectively, were transformed under optimal conditions of 60 degrees C, pH 5, and 5 mmol l(-1) CuCl(2) after a 24-h incubation period. Other PAHs including fluorene, acenaphthylene, phenanthrene, and benzo[a]anthracene were also oxidized by the crude CueO. These findings indicated the potential application of prokaryotic laccases in enhancing the mineralization of benzo[a]pyrene by PAH-degrading bacteria.
收录类别	SCI
WOS关键词	ASCOMYCETE MELANOCARPUS-ALBOMYCES ; FUNGUS PLEUROTUS-OSTREATUS ; MULTICOPPER OXIDASE CUEO ; ESCHERICHIA-COLI ; TRAMETES-VERSICOLOR ; BACILLUS-SUBTILIS ; ELECTRON-TRANSFER ; CRYSTAL-STRUCTURE ; ENDOSPORE COAT ; PURIFICATION
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000288333800018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558960
专题	南京土壤研究所
通讯作者	Lin, Xiangui
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Dept Biol & Biochem, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 3.Hongkong Baptist Univ, Joint Open Lab Soil & Environm, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.Hong Kong Baptist Univ, Croucher Inst Environm Sci, Dept Biol, Kowloon Tong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Jun,Lin, Xiangui,Zhang, Jing,et al. Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,89(6):1841-1849.
APA	Zeng, Jun,Lin, Xiangui,Zhang, Jing,Li, Xuanzhen,&Wong, Ming Hung.(2011).Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,89(6),1841-1849.
MLA	Zeng, Jun,et al."Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 89.6(2011):1841-1849.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。