QQ客服 官方微博 反馈留言

	Effects of copper on methane emission, methanogens and methanotrophs in the rhizosphere and bulk soil of rice paddy
	Mao, Ting-Ting; Yin, Rui; Deng, Huan
刊名	CATENA
	2015-10-01
卷号	133页码:233-240
关键词	Greenhouse gas mcrA gene pmoA gene Rhizosphere Methylosarcina
ISSN号	0341-8162
DOI	10.1016/j.catena.2015.05.024
通讯作者	Deng, Huan(hdeng@njnu.edu.cn)
英文摘要	Copper contamination is common in paddy fields due to wastewater irrigation and application of sludge and Cu-containing fungicides. We aimed to study the effects of copper (Cu2+) application on methane emission, methanogens and methanotrophs in both the rhizosphere and bulk soil. The study was conducted in rhizobox in which flooded soil was applied with different Cu2+ concentrations. Methane emission was collected with static chamber method and determined by gas chromatography. The diversity and composition of methanogens and methanotrophs were studied using PCR DGGE and sequencing analysis of methanogenic 165 rRNA and pmoA genes. The abundance of methanogens and methanotrophs was determined using quantitative real-time PCR of mcrA and pmoA genes, respectively. The results showed that Cu2+ application decreased methane emission along with the diversity and abundance of methanogens and methanotrophs, although the application of 200 mg kg(-1) Cu2+ did not significantly decrease the diversity of methanogens and methanotrophs in the rhizosphere. In addition, Cu2+ decreased methanotrophs diversity more profoundly than methanogens diversity. Methanogens in both the rhizosphere and bulk soil were closely related to Methanosaeta, Methanosarcina, Methanobacterium and Methanomicrobia archaeon. Methanotrophs in rhizosphere soil were clustered into four groups (type I methanotrophs, Methylobacter, Methylomonas and Methylosarcina) while those in bulk soil were much less diverse. The addition of 200 to 800 mg kg(-1) Cu2+ did not dramatically change the composition of methanogens; however, for methanotrophs, only one DGGE band belonging to Methylosarcina was present after the addition of 800 mg kg(-1) Cu2+. We conclude that methanotrophs were more sensitive to Cu2+ addition than methanogens, and that the rhizosphere environment alleviated Cu2+ stress on methanogens and methanotrophs. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
WOS关键词	FIELD SOIL ; RIBOSOMAL-RNA ; MICROBIAL COMMUNITIES ; POPULATION-DYNAMICS ; PLANTS ; BACTERIA ; ARCHAEA ; MCRA ; CH4 ; PCR
WOS研究方向	Geology ; Agriculture ; Water Resources
WOS类目	Geosciences, Multidisciplinary ; Soil Science ; Water Resources
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000359960600024
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558334
专题	南京土壤研究所
通讯作者	Deng, Huan
作者单位	Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Mao, Ting-Ting,Yin, Rui,Deng, Huan. Effects of copper on methane emission, methanogens and methanotrophs in the rhizosphere and bulk soil of rice paddy[J]. CATENA,2015,133:233-240.
APA	Mao, Ting-Ting,Yin, Rui,&Deng, Huan.(2015).Effects of copper on methane emission, methanogens and methanotrophs in the rhizosphere and bulk soil of rice paddy.CATENA,133,233-240.
MLA	Mao, Ting-Ting,et al."Effects of copper on methane emission, methanogens and methanotrophs in the rhizosphere and bulk soil of rice paddy".CATENA 133(2015):233-240.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。