| Integrated regulation triggered by a cryophyte omega-3 desaturase gene confers multiple-stress tolerance in tobacco | |
| Shi, Yulan1; Yue, Xiule2; An, Lizhe1,2 | |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 2018-04-03 | |
| 卷号 | 69期号:8页码:2131-2148 |
| 关键词 | omega-3 FAD gene Ca2+ signaling Chorispora bungeana multiple-stress tolerance ROS stress-responsive gene |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/ery050 |
| 通讯作者 | Shi, Yulan(sylbaby15@lzb.ac.cn) |
| 英文摘要 | omega-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through linolenic acid (C18: 3)-induced membrane stabilization, but a comprehensive analysis of their roles in stress adaptation is lacking. Here, we isolated a microsomal omega-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bungeana) and elucidated its functions in stress tolerance. CbFAD3, exhibiting a high identity to Arabidopsis AtFAD3, was up-regulated by abiotic stresses. Its functionality was verified by heterogonous expression in yeast. Overexpression of CbFAD3 in tobacco constitutively increased C18: 3 in both leaves and roots, which maintained the membrane fluidity, and enhanced plant tolerance to cold, drought, and salt stresses. Notably, the constitutively increased C18: 3 induced a sustained activation of plasma membrane Ca2+-ATPase, thereby, changing the stress-induced Ca2+ signaling. The reactive oxygen species (ROS) scavenging system, which was positively correlated with the level of C18: 3, was also activated in the transgenic lines. Microarray analysis showed that CbFAD3-overexpressing plants increased the expression of stressresponsive genes, most of which are affected by C18: 3, Ca2+, or ROS. Together, CbFAD3 confers tolerance to multiple stresses in tobacco through the C18: 3-induced integrated regulation of membrane, Ca2+, ROS, and stress-responsive genes. This is in contrast with previous observations that simply attribute stress tolerance to membrane stabilization. |
| 收录类别 | SCI |
| WOS关键词 | PLASMA-MEMBRANE CA2+-ATPASE ; SUSPENSION-CULTURED CELLS ; FATTY-ACID DESATURATION ; TIME RT-PCR ; ABIOTIC STRESS ; OMEGA-3-FATTY-ACID DESATURASE ; CHORISPORA-BUNGEANA ; ARABIDOPSIS-THALIANA ; TRANSGENIC TOBACCO ; MOLECULAR-CLONING |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000430092100026 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2558140 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Shi, Yulan |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Extreme Stress Resistance & Biotechnol Lab, Lanzhou 73000, Gansu, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 73000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yulan,Yue, Xiule,An, Lizhe. Integrated regulation triggered by a cryophyte omega-3 desaturase gene confers multiple-stress tolerance in tobacco[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(8):2131-2148. |
| APA | Shi, Yulan,Yue, Xiule,&An, Lizhe.(2018).Integrated regulation triggered by a cryophyte omega-3 desaturase gene confers multiple-stress tolerance in tobacco.JOURNAL OF EXPERIMENTAL BOTANY,69(8),2131-2148. |
| MLA | Shi, Yulan,et al."Integrated regulation triggered by a cryophyte omega-3 desaturase gene confers multiple-stress tolerance in tobacco".JOURNAL OF EXPERIMENTAL BOTANY 69.8(2018):2131-2148. |
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