Radix Angelicae Sinensis and Radix Hedysari enhance radiosensitivity of C-12(6+) radiation in human liver cancer cells by modulating apoptosis protein | |
Kou, Wei1; Li, Ying-Dong1,2; Liu, Kai2; Sun, Shao-Bo2; Dong, Yu-Mei2; Wu, Zhen-Hua3,4 | |
刊名 | SAUDI MEDICAL JOURNAL
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2014-09-01 | |
卷号 | 35期号:9页码:945-952 |
ISSN号 | 0379-5284 |
通讯作者 | Li, Ying-Dong(yxkw@xbmu.edu.cn) |
英文摘要 | Objectives: To investigate the radiosensitizing effects of Radix Angelicae Sinensis-Radix Hedysari (RAS-RH [an ultra-filtration extract]) and its underlying mechanisms in human liver cancer cells H22. Methods: This study was conducted between September 2010 and August 2012 in the Gansu University of Traditional Chinese Medicine, Lanzhou, China. The groups were assigned as the control group, drug (RAS-RH) group, C-12(6+) radiation group, and combination group. The cell counting kit-8 assay, colony formation assay, cell cycle changes, and apoptosis analysis were carried out, and survivin and casepase-9 were evaluated by reverse transcription polymerase chain reaction and Western blot analyses in the 4 groups. Results: The inhibitory effect of RAS-RH on H22 cells was dependent on both concentration and time, RAS-RH was able to enhance the radiosensitivity of H22 cells by increasing cell survival fraction and radiosensitization parameters. Apoptosis and the gap2/mitosis (G2/M) phase transition induced by C-12(6+) heavy ion radiation was enhanced by RAS-RH treatment. Irradiation, combined with RAS-RH, decreased survivin expression while increasing casepase-9 expression in H22 cells. Conclusion: The RAS-RH increased the radiosensitivity of H22 cells of C-12(6+) heavy ion radiation significantly, and its possible mechanism of radiosensitization is to enhance caspase-dependent apoptosis through the down-regulation of survivin, thus, it can be used as an effective radiosensitizer. |
收录类别 | SCI |
WOS关键词 | ENERGETIC HEAVY-IONS ; HEPATOCELLULAR-CARCINOMA ; SIGNAL-TRANSDUCTION ; SURVIVIN GENE ; CYCLE ; ACTIVATION ; EXPRESSION ; CASPASE-9 ; POLYMORPHISMS ; FIBROBLASTS |
WOS研究方向 | General & Internal Medicine |
WOS类目 | Medicine, General & Internal |
语种 | 英语 |
出版者 | SAUDI MED J |
WOS记录号 | WOS:000343245700002 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2557864 |
专题 | 寒区旱区环境与工程研究所 |
通讯作者 | Li, Ying-Dong |
作者单位 | 1.Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab TCM Prevent & Control Chron Dis Gansu Pro, Lanzhou, Peoples R China 3.Chinese Acad Sci, Gansu Coll Tradit Chinese Med, Lanzhou, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Kou, Wei,Li, Ying-Dong,Liu, Kai,et al. Radix Angelicae Sinensis and Radix Hedysari enhance radiosensitivity of C-12(6+) radiation in human liver cancer cells by modulating apoptosis protein[J]. SAUDI MEDICAL JOURNAL,2014,35(9):945-952. |
APA | Kou, Wei,Li, Ying-Dong,Liu, Kai,Sun, Shao-Bo,Dong, Yu-Mei,&Wu, Zhen-Hua.(2014).Radix Angelicae Sinensis and Radix Hedysari enhance radiosensitivity of C-12(6+) radiation in human liver cancer cells by modulating apoptosis protein.SAUDI MEDICAL JOURNAL,35(9),945-952. |
MLA | Kou, Wei,et al."Radix Angelicae Sinensis and Radix Hedysari enhance radiosensitivity of C-12(6+) radiation in human liver cancer cells by modulating apoptosis protein".SAUDI MEDICAL JOURNAL 35.9(2014):945-952. |
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