QQ客服 官方微博 反馈留言

	Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
	Li, Ming1,2; Liang, Zhaoxu1; Di, Cuixia3; Fang, Weikuan4; Wu, Kaichao1; Chen, Maoshan5; He, Shanshan1; Zeng, Yuan4; Jing, Yan1; Liang, Jun1
刊名	BIOMED RESEARCH INTERNATIONAL
	2016
页码	11
ISSN号	2314-6133
DOI	10.1155/2016/2483258
通讯作者	An, Lizhe(lizhean@lzu.edu.cn)
英文摘要	Background. Water channel proteins, also called a quaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.
收录类别	SCI
WOS关键词	SUSPENSION-CULTURED CELLS ; MAJOR INTRINSIC PROTEINS ; CHILLING TOLERANCE ; XENOPUS-OOCYTES ; PLANT AQUAPORINS ; PLASMA-MEMBRANE ; EXPRESSION ; CLONING ; FAMILY ; MERCURY
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
语种	英语
出版者	HINDAWI PUBLISHING CORP
WOS记录号	WOS:000383094400001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2557460
专题	寒区旱区环境与工程研究所
通讯作者	An, Lizhe
作者单位	1.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China 5.La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic 3086, Australia 6.Lanzhou Univ, State Key Lab Arid Agroecol, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Ming,Liang, Zhaoxu,Di, Cuixia,et al. Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana[J]. BIOMED RESEARCH INTERNATIONAL,2016:11.
APA	Li, Ming.,Liang, Zhaoxu.,Di, Cuixia.,Fang, Weikuan.,Wu, Kaichao.,...&An, Lizhe.(2016).Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana.BIOMED RESEARCH INTERNATIONAL,11.
MLA	Li, Ming,et al."Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana".BIOMED RESEARCH INTERNATIONAL (2016):11.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。