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	Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis
	Wang Lirong1; Zhao Pengshan2; Zhao Xin2; Wang Xiaopeng3; Ma Xiaofei2; Li Yi1
刊名	ACTA SOCIETATIS BOTANICORUM POLONIAE
	2016
卷号	85期号:1页码:14
关键词	Braya humilis drought polyethylene glycol physiological response BhCIPK12 expression pattern
ISSN号	0001-6977
DOI	10.5586/asbp.3487
通讯作者	Li Yi(liyi@gsau.edu.cn)
英文摘要	Braya humilis (Brassicaceae) is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s) mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG)-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR), cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism(s) mediating drought tolerance in B. humilis.
收录类别	SCI
WOS关键词	TRITICUM-AESTIVUM L. ; DROUGHT STRESS ; ANTIOXIDANT DEFENSE ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; TOLERANCE ; LEAVES ; IDENTIFICATION ; ARABIDOPSIS ; EXPRESSION
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	POLSKIE TOWARZYSTWO BOTANICZNE
WOS记录号	WOS:000374192800002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2557296
专题	寒区旱区环境与工程研究所
通讯作者	Li Yi
作者单位	1.Gansu Agr Univ, Coll Forestry Sci, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Teachers Coll, Dept Geog, Dingxi 743000, Peoples R China
推荐引用方式 GB/T 7714	Wang Lirong,Zhao Pengshan,Zhao Xin,et al. Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis[J]. ACTA SOCIETATIS BOTANICORUM POLONIAE,2016,85(1):14.
APA	Wang Lirong,Zhao Pengshan,Zhao Xin,Wang Xiaopeng,Ma Xiaofei,&Li Yi.(2016).Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis.ACTA SOCIETATIS BOTANICORUM POLONIAE,85(1),14.
MLA	Wang Lirong,et al."Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis".ACTA SOCIETATIS BOTANICORUM POLONIAE 85.1(2016):14.

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