| A rapid immunochromatographic test to detect the lily mottle virus | |
| Zhang, Yubao1; Wang, Yajun1; Yang, Wanrong2; Xie, Zhongkui1; Wang, Ruoyu1; Kutcher, Hadley Randal3; Guo, Zhihong1 | |
| 刊名 | Journal of virological methods
 |
| 2015-08-01 | |
| 卷号 | 220页码:43-48 |
| 关键词 | Immunochromatographic strip Lmov Pcr Rapid detection |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2015.04.010 |
| 通讯作者 | Xie, zhongkui(wxhcas@lzb.ac.cn) |
| 英文摘要 | We developed a rapid immunochromatographic strip (ics) test for lily mottle virus (lmov). the test is based on a double-antibody sandwich format and employs two distinct anti-lmov polyclonal antibodies (igg(3) and igg(4)). the first antibody, igg(3) was conjugated with colloidal gold, and the second antibody, igg(4) was used as the capture antibody at the test line. the performance of the ics test was evaluated and the results obtained were compared with a quadruplex rt-pcr assay. when serial dilutions of purified lmov were tested, the lmov detection limit of the ics test was 8.0 x 10(-9) mg/ml, which was in complete agreement with the results of quadruplex rt-pcr. compared with quadruplex rt-pcr, the specificity and sensitivity of ics were 98.7 and 100%, respectively. there was therefore significant agreement between the results obtained from the two tests (kappa = 0.982). the ics test therefore appears to be broadly applicable, and will be especially useful in the field, as well as in areas without laboratory facilities, to support efforts to detect and control lmov. (c) 2015 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | DAVIDII VAR. UNICOLOR ; SYMPTOMLESS VIRUS ; LILIUM ; POTYVIRUS ; PROTEINS ; STRIP |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000356112300009 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2555019 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Xie, Zhongkui |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Ningxia Agr Comprehens Dev Off, Yinchuan 750002, Peoples R China 3.Univ Saskatchewan, Coll Agr & Bioresources, Saskatoon, SK S7N 5A8, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Yubao,Wang, Yajun,Yang, Wanrong,et al. A rapid immunochromatographic test to detect the lily mottle virus[J]. Journal of virological methods,2015,220:43-48. |
| APA | Zhang, Yubao.,Wang, Yajun.,Yang, Wanrong.,Xie, Zhongkui.,Wang, Ruoyu.,...&Guo, Zhihong.(2015).A rapid immunochromatographic test to detect the lily mottle virus.Journal of virological methods,220,43-48. |
| MLA | Zhang, Yubao,et al."A rapid immunochromatographic test to detect the lily mottle virus".Journal of virological methods 220(2015):43-48. |
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