Mutation spectrum in Salmonella induced by environmental tobacco smoke

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	Mutation spectrum in Salmonella induced by environmental tobacco smoke
	Zhou, RQ; Yu, G; Zhou, YQ; Zeng, XF; Zhou, RQ, Liaoning Univ, Dept Biol, Shenyang 110036, Liaoning Prov, Peoples R China
刊名	ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
	1999
卷号	33 期号:3 页码:185-193
关键词	Environmental Tobacco Smoke Mutation Spectrum Ames/salmonella
ISSN号	0893-6692
英文摘要	Environmental tobacco smoke (ETS) is a major source of indoor air pollution. Extractable-respirable particulate (ERP) from the ETS-contaminated indoor air (ERP-ETS) was collected from six passenger train cars and one control room. The mutagenicity of ERP-ETS was tested in the Ames/Salmonella test in the presence of male rat liver microsomal Fraction S9. The mutation spectrum of ERP-ETS was determined by colony probe hybridization and polymerase chain reaction/DNA sequence analysis in approximately 2,370 His(+) revertants. The results indicate that the majority of ERP-ETS-induced mutations were a two-base deletion of GC or CG within the hotspot sequence of CGCGCGCG at the frameshift hisD3052 allele in strain TA98, The ERP-ETS From the control room induced approximately 94.3% such deletions, while the ERP-ETS collected From the passenger cars induced approximately 89.6% such deletions. The ERP-ETS either from the control room or from the passenger cars induced approximately 74%C/G --> A/T transversions, and approximately 23% C/G --> T/A transitions within the primary target CCC at the hisG46 allele in strain TA100. (C) 1999 Wiley-Liss, Inc.
学科主题	Environmental Sciences ; Genetics & Heredity ; Toxicology
语种	英语
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55613]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhou, RQ, Liaoning Univ, Dept Biol, Shenyang 110036, Liaoning Prov, Peoples R China
推荐引用方式 GB/T 7714	Zhou, RQ,Yu, G,Zhou, YQ,et al. Mutation spectrum in Salmonella induced by environmental tobacco smoke[J]. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS,1999,33(3):185-193.
APA	Zhou, RQ,Yu, G,Zhou, YQ,Zeng, XF,&Zhou, RQ, Liaoning Univ, Dept Biol, Shenyang 110036, Liaoning Prov, Peoples R China.(1999).Mutation spectrum in Salmonella induced by environmental tobacco smoke.ENVIRONMENTAL AND MOLECULAR MUTAGENESIS,33(3),185-193.
MLA	Zhou, RQ,et al."Mutation spectrum in Salmonella induced by environmental tobacco smoke".ENVIRONMENTAL AND MOLECULAR MUTAGENESIS 33.3(1999):185-193.