Toxicity and distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China

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	Toxicity and distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China
	Xia, GL; Wu, ZL; Yang, Y; Xie, LP; Hu, JC; Wang, SJ; Zhang, RQ; Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
刊名	TOXICON
	2005
卷号	46 期号:4 页码:471-476
关键词	Tetrodotoxin Tetrodotoxin-producing Bacteria Fugu Rubripes
ISSN号	0041-0101
英文摘要	To investigate the relationship between the toxicity of puffer fish and the distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China, bacteria were isolated from each organ (ovaries, livers, intestines and gallbladders) and screened for tetrodotoxin (TTX) production. 20 out of 36 isolated strains were found to produce TTX in vitro. In the organs of ovaries and livers whose toxicity is more potent than other organs, the number and toxicity of TTX-producing strains was greater than that of others. Most TTX-producing bacterial strains were identified as Bacillus spp. (19 strains) and Actinomycete spp. (1 strain) based on the morphological observation, physiological and biochemical characteristics and G+C content of DNA. The purified toxin was identified to be TTX by high performance liquid chromatography assay, thin-layer chromatography assay and electrospray ionization mass spectrometry analysis. Our results suggested that TTX-producing bacteria are closely related to the toxification of the puffer fish. More research is needed to elucidate the mechanism of TTX synthesis and the role of TTX in bacteria. (C) 2005 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy ; Toxicology
语种	英语
WOS记录号	WOS:000232127100014
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55065]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Xia, GL,Wu, ZL,Yang, Y,et al. Toxicity and distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China[J]. TOXICON,2005,46(4):471-476.
APA	Xia, GL.,Wu, ZL.,Yang, Y.,Xie, LP.,Hu, JC.,...&Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China.(2005).Toxicity and distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China.TOXICON,46(4),471-476.
MLA	Xia, GL,et al."Toxicity and distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China".TOXICON 46.4(2005):471-476.