Genetic diversity in the mycoparasite Sphaerellopsis filum inferred from AFLP analysis and ITS-5.8S sequences

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	Genetic diversity in the mycoparasite Sphaerellopsis filum inferred from AFLP analysis and ITS-5.8S sequences
	Bayon, C; Yuan, ZW; Ruiz, C; Liesebach, M; Pei, MH; Pei, MH, Rothamsted Res, Harpenden AL5 1JQ, Herts, England
刊名	MYCOLOGICAL RESEARCH
	2006
卷号	110 页码:1200-1206
关键词	Eudarluca Caricis Genetic Diversity Melampsora Populus Salix
ISSN号	0953-7562
英文摘要	Genetic diversity among 51 isolates of the mycoparasite Sphaerellopsisfilurn from Melampsora rusts on willow and poplar was examined using AFLP. Genetic variation was relatively low among the isolates (Nei & Li's similarities >= 90). Genetic diversity calculated using Shannon index was 0.119 at Loughgall, Northern Ireland, 0.109 at Markington, northern England, 0.039 at Craibstone, Scotland, and 0.015 at Long Ashton, southwest England. At Long Ashton, 14 out of 16 isolates shared the same AFLP bands. Two genotypes were found at both Markington and Loughgall. The low genetic diversity and a high rate of clonality suggested that asexual reproduction plays a major role in S. filum epidemics. Sequence information was also obtained from the ITS-5.8S region of the ribosomal DNA from the S. filum isolates derived from willow and poplar rusts and six isolates derived from other sources. ITS sequences were identical among all the 51 isolates from willow and poplar rusts. ITS analysis placed S. filum isolates from Melampsora spp. on willow and poplar, Puccinia coronata on grass and Melampsora sp. on Euphorbia sp. into one clade and the isolates from blackberry rust Phragmidium violaceum and larch rust Triphragmiopsis laricinum into another. Nucleotide sequence differences between the two groups were 8.4-10.4 %. The ITS-5.8S sequences obtained in this study were compared with those deposited in the GenBank database. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
学科主题	Mycology
语种	英语
WOS记录号	WOS:000242428700009
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54809]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Pei, MH, Rothamsted Res, Harpenden AL5 1JQ, Herts, England
推荐引用方式 GB/T 7714	Bayon, C,Yuan, ZW,Ruiz, C,et al. Genetic diversity in the mycoparasite Sphaerellopsis filum inferred from AFLP analysis and ITS-5.8S sequences[J]. MYCOLOGICAL RESEARCH,2006,110:1200-1206.
APA	Bayon, C,Yuan, ZW,Ruiz, C,Liesebach, M,Pei, MH,&Pei, MH, Rothamsted Res, Harpenden AL5 1JQ, Herts, England.(2006).Genetic diversity in the mycoparasite Sphaerellopsis filum inferred from AFLP analysis and ITS-5.8S sequences.MYCOLOGICAL RESEARCH,110,1200-1206.
MLA	Bayon, C,et al."Genetic diversity in the mycoparasite Sphaerellopsis filum inferred from AFLP analysis and ITS-5.8S sequences".MYCOLOGICAL RESEARCH 110(2006):1200-1206.