Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis

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	Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis
	Li, XY; Zhang, HW; Wu, MN; Zhang, Y; Zhang, CG; Zhang, HW, Chinese Acad Sci, Microbial Resources & Ecol Grp Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2008
卷号	20 期号:5 页码:619-625
关键词	Methamidophos Clone Sequencing Dgge (Denaturing Gradient Gel Electrophoresis) Fungi Diversity
ISSN号	1001-0742
英文摘要	Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DGGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.
学科主题	Environmental Sciences
语种	英语
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54465]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, HW, Chinese Acad Sci, Microbial Resources & Ecol Grp Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Li, XY,Zhang, HW,Wu, MN,et al. Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(5):619-625.
APA	Li, XY,Zhang, HW,Wu, MN,Zhang, Y,Zhang, CG,&Zhang, HW, Chinese Acad Sci, Microbial Resources & Ecol Grp Inst Appl Ecol, Shenyang 110016, Peoples R China.(2008).Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(5),619-625.
MLA	Li, XY,et al."Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.5(2008):619-625.