Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site

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	Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site
	Zhang, XX; Jia, YF; Wang, X; Xu, LY; Jia, YF, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2008
卷号	20 期号:12 页码:1501-1507
关键词	Microbial Reduction Arsenic Phylogenetic Analysis
ISSN号	1001-0742
英文摘要	Microbial reduction of As(V) (i.e., arsenate) plays an important role in arsenic (As) mobilization in aqueous environment. In this study, we investigated As(V) reduction characteristics of the bacteria enriched from the arsenic-contaminated soil at an abandoned smelter site. It was found that As(V) was completely reduced to As(III) (i.e., arsenite) in 21 h. After 3-d incubation, a yellow solid was precipitated and the concentration of As(HI) decreased sharply. After 150 h incubation, ca. 65% of soluble arsenic was removed from the solution. The analysis of the precipitate by scanning electron microscopy and energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD) revealed that the main component was crystalline arsenic sulfide (AsS). Microbial mediated reduction and mobilization of adsorbed As(V) on ferric hydroxide was also examined. In the microcosm slurry experiment, ca. 53% of the adsorbed As(V) was reduced to As(111) by the bacteria, which resulted in an appreciable release of arsenic into aqueous phase. The released arsenic was present predominantly as As(III). The microbial diversity was analyzed by 16S rDNA-dependent molecular phylogeny. A near-full-length 16S rDNA gene clone library was constructed. The 197 clones were analyzed using RFLP (restriction fragment length polymorphism) and 72 OTUs were obtained, which contributed 51 % of the content for total clone number in six OTUs. Six bacterial clones in these six OTUs were selected for sequencing and the sequenced clones were found to belong to the group Caloramator, Clostridium, and Bacillus.
学科主题	Environmental Sciences
语种	英语
WOS记录号	WOS:000261835500016
资助机构	National Natural Science Foundation of China [40673079, 40773076]
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54291]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Jia, YF, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhang, XX,Jia, YF,Wang, X,et al. Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(12):1501-1507.
APA	Zhang, XX,Jia, YF,Wang, X,Xu, LY,&Jia, YF, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2008).Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(12),1501-1507.
MLA	Zhang, XX,et al."Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.12(2008):1501-1507.