Biological analysis of the deletion mutants of Staphylococcal enterotoxin C2

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	Biological analysis of the deletion mutants of Staphylococcal enterotoxin C2
	Wang, XG; Zhang, HW; Xu, MK; Liu, CX; Zhang, CG; Xu, MK, Chinese Acad Sci, Microbiol Grp, Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2009
卷号	83 期号:6 页码:1077-1084
关键词	Staphylococcal Enterotoxin C2 Superantigen Activity Fever Activity Immunotherapeutic Agent
ISSN号	0175-7598
英文摘要	To investigate the functional domains involved in the biological activity of staphylococcal enterotoxin (SEC2), a series of SEC2 mutants were constructed. Deletion of the last 77 amino acids at the C-terminus of SEC2 did not affect its native superantigen and fever activities, and further removal of the C-terminal residues reduced SEC2 activities significantly. On the other hand, the mutants lacking 18 or more N-terminal residues severely impaired superantigen activity. These data indicated that the functional regions for the biological activities of SEC2 were confined to N-terminal domain, further implied that the proper three-dimensional structure of SEC2 is not needed for its biological activities. Our results deliver valuable information that it is possible to design new SEC2 immunotherapeutic agents which have the superantigen activity and low molecular weight for permeability.
学科主题	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000267679900010
资助机构	Institute of Applied Ecology of the CAS [06LYQYC001, 08SBS111S3]
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54107]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Xu, MK, Chinese Acad Sci, Microbiol Grp, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Wang, XG,Zhang, HW,Xu, MK,et al. Biological analysis of the deletion mutants of Staphylococcal enterotoxin C2[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2009,83(6):1077-1084.
APA	Wang, XG,Zhang, HW,Xu, MK,Liu, CX,Zhang, CG,&Xu, MK, Chinese Acad Sci, Microbiol Grp, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2009).Biological analysis of the deletion mutants of Staphylococcal enterotoxin C2.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,83(6),1077-1084.
MLA	Wang, XG,et al."Biological analysis of the deletion mutants of Staphylococcal enterotoxin C2".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 83.6(2009):1077-1084.