Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils

doi:10.1186/s40168-019-0647-0

	Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils
	Zhao, Zhi-Bo 1,2; He, Ji-Zheng 1,2,3; Geisen, Stefan 4; Han, Li-Li 1,2; Wang, Jun-Tao 1; Shen, Ju-Pei 1,2; Wei, Wen-Xue 5; Fang, Yun-Ting 6; Li, Pei-Pei 7; Zhang, Li-Mei 1,2
刊名	MICROBIOME
	2019-02-27
卷号	7 页码:16
关键词	Soil protists Microbiome Soil type Nitrogen fertilizers High-throughput sequencing
ISSN号	2049-2618
DOI	10.1186/s40168-019-0647-0
英文摘要	Background: Agricultural food production is at the base of food and fodder, with fertilization having fundamentally and continuously increased crop yield over the last decades. The performance of crops is intimately tied to their microbiome as they together form holobionts. The importance of the microbiome for plant performance is, however, notoriously ignored in agricultural systems as fertilization disconnects the dependency of plants for often plant-beneficial microbial processes. Moreover, we lack a holistic understanding of how fertilization regimes affect the soil microbiome. Here, we examined the effect of a 2-year fertilization regime (no nitrogen fertilization control, nitrogen fertilization, and nitrogen fertilization plus straw amendment) on entire soil microbiomes (bacteria, fungi, and protist) in three common agricultural soil types cropped with maize in two seasons. Results: We found that the application of nitrogen fertilizers more strongly affected protist than bacterial and fungal communities. Nitrogen fertilization indirectly reduced protist diversity through changing abiotic properties and bacterial and fungal communities which differed between soil types and sampling seasons. Nitrogen fertilizer plus straw amendment had greater effects on soil physicochemical properties and microbiome diversity than nitrogen addition alone. Moreover, nitrogen fertilization, even more together with straw, increased soil microbiome network complexity, suggesting that the application of nitrogen fertilizers tightened soil microbiomes interactions. Conclusions: Together, our results suggest that protists are the most susceptible microbiome component to the application of nitrogen fertilizers. As protist communities also exhibit the strongest seasonal dynamics, they serve as the most sensitive bioindicators of soil changes. Changes in protist communities might have long-term effects if some of the key protist hubs that govern microbiome complexities as top microbiome predators are altered. This study serves as the stepping stone to promote protists as promising agents in targeted microbiome engineering to help in reducing the dependency on exogenous unsustainably high fertilization and pesticide applications.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB15020200] ; National Key RD Program[2017YFD0200600] ; NWO-VENI grant from the Netherlands Organisation for Scientific Research[016.Veni.181.078] ; Youth Innovation Promotion Association, Chinese Academy of Sciences
WOS研究方向	Microbiology
语种	英语
出版者	BMC
WOS记录号	WOS:000462993500001
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/123995]
专题	中国科学院沈阳应用生态研究所
通讯作者	Geisen, Stefan; Zhang, Li-Mei
作者单位	1.Chinese Acad Sci, State Key Lab Urban & Reg Ecol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic 3010, Australia 4.Netherlands Inst Ecol NIOO KNAW, Dept Terr Ecol, NL-6708 PB Wageningen, Netherlands 5.Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg & Taoyuan Stn A, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Forest Ecol & Management, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China 7.Henan Agr Univ, Coll Resource & Environm Sci, Zhengzhou 450002, Henan, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Zhi-Bo,He, Ji-Zheng,Geisen, Stefan,et al. Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils[J]. MICROBIOME,2019,7:16.
APA	Zhao, Zhi-Bo.,He, Ji-Zheng.,Geisen, Stefan.,Han, Li-Li.,Wang, Jun-Tao.,...&Zhang, Li-Mei.(2019).Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils.MICROBIOME,7,16.
MLA	Zhao, Zhi-Bo,et al."Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils".MICROBIOME 7(2019):16.