Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method

doi:10.3139/113.110429

	Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method
	Han, Siqin 2; Zhang, Jie 1; Ma, Fang 1; Zhang, Ying 2; Zhao, Feng 1; Liang, Xiaolong 2; Ban, Yunhe 3
刊名	TENSIDE SURFACTANTS DETERGENTS
	2016-05-01
卷号	53 期号:3 页码:243-248
关键词	Rhamnolipid Quantification Oil Spreading Method Optimization
ISSN号	0932-3414
DOI	10.3139/113.110429
英文摘要	A rapid and reliable method to quantify rhamnolipid is indispensable to study and evaluate rhamnolipid-producing bacteria. Five methods were attempted to quantify rhamnolipid in bacteria culture. The oil spreading method better predicted the rhamnolipid concentration and is simpler than the other methods. The potential influencing factors (temperature, pH, salinity, metals, bacterial cells, carbon sources) were investigated. The common substance in fermentation broth has little effect on oil spreading circle. An optimized protocol of the oil spreading method to quantify rhamnolipid was proposed. A positive linear correlation (R-2= 0.9908) was established between the diameter of the formed oil spreading circle and the concentration of rhamnolipid (100-800 mg L-1). Results of a large number of samples suggested that the oil spreading method is easy, rapid and reliable to analyze quantitatively rhamnolipids.
WOS研究方向	Chemistry ; Engineering
语种	英语
出版者	CARL HANSER VERLAG
WOS记录号	WOS:000375749300004
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122635]
专题	中国科学院沈阳应用生态研究所
通讯作者	Ma, Fang
作者单位	1.Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Sch Municipal & Environm Engn, Harbin 150090, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 3.Shenyang Normal Univ, Coll Chem & Life Sci, Shenyang, Peoples R China
推荐引用方式 GB/T 7714	Han, Siqin,Zhang, Jie,Ma, Fang,et al. Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method[J]. TENSIDE SURFACTANTS DETERGENTS,2016,53(3):243-248.
APA	Han, Siqin.,Zhang, Jie.,Ma, Fang.,Zhang, Ying.,Zhao, Feng.,...&Ban, Yunhe.(2016).Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method.TENSIDE SURFACTANTS DETERGENTS,53(3),243-248.
MLA	Han, Siqin,et al."Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method".TENSIDE SURFACTANTS DETERGENTS 53.3(2016):243-248.