Nocardiopsis oceani sp nov and Nocardiopsis nanhaiensis sp nov., actinomycetes isolated from marine sediment of the South China Sea

doi:10.1099/ijsem.0.000425

	Nocardiopsis oceani sp nov and Nocardiopsis nanhaiensis sp nov., actinomycetes isolated from marine sediment of the South China Sea
	Pan, Hua-Qi 1; Zhang, Dao-Feng 2,3,4; Li, Li 5; Jiang, Zhao 4; Cheng, Juan 4; Zhang, Yong-Guang 5; Wang, Hong-Fei 5; Hu, Jiang-Chun 1; Li, Wen-Jun 2,3,4,5
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2015-10-01
卷号	65 页码:3384-3391
ISSN号	1466-5026
DOI	10.1099/ijsem.0.000425
英文摘要	Two actinomycete strains, designated 10A08A(T) and 10A08B(T), were isolated from marine sediment samples of the South China Sea and their taxonomic positions were determined by a polyphasic approach. The two Gram-stain-positive, aerobic strains produced branched substrate mycelium and aerial hyphae, and no diffusible pigment was produced in the media tested. At maturity, spore chains were formed on aerial hyphae and all mycelium fragmented with age. Whole-cell hydrolysates of both strains contained meso-diaminopimelic acid and no diagnostic sugars. Their predominant menaquinones (>10 %) were MK-9(H-4), MK-9(H-6) and MK-10(H-6) for strain 10A08A(T) and MK-9(H-4), MK-9(H-6), MK-10(H-6) and MK-10(H-6) for strain 10A08B(T). The polar lipids detected from the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unknown phosphoglycolipids and phospholipids. The major fatty acids (>10 %) of both strains were iso-C-(16) (: 0) and summed feature 4 (iso-C-17 : 1 I and/or anteiso-C-17 : (1) B). The genomic DNA G + C contents of strains 10A08A(T) and 10A08B(T) were 70.9 and 71.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarities, the two strains were shown to be most closely related to species of the genus Nocardiopsis. DNA DNA hybridization relatedness values of <70 % between these two isolates and their closest neighbour, Nocardiopsis terrae YIM 90022(T), and between the two strains supported the conclusion that they represent two novel species. Based on phylogenetic analysis and phenotypic and genotypic data, it is concluded that the two isolates belong to the genus Nocardiopsis, and the names Nocardiopsis oceani sp. nov. (type strain 10A08A(T)=DSM 45931(T)=BCRC 16951(T)) and Nocardiopsis nanhaiensis sp. nov. (type strain 10A08B(T)=CGMCC 47227(T)=BCRC 16952(T)) are proposed.
WOS研究方向	Microbiology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000366152100024
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122323]
专题	中国科学院沈阳应用生态研究所
通讯作者	Li, Wen-Jun
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Sun Yat Sen Univ, Coll Ecol & Evolut, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China 3.Sun Yat Sen Univ, Coll Ecol & Evolut, Guangdong Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China 4.Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Peoples R China 5.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China
推荐引用方式 GB/T 7714	Pan, Hua-Qi,Zhang, Dao-Feng,Li, Li,et al. Nocardiopsis oceani sp nov and Nocardiopsis nanhaiensis sp nov., actinomycetes isolated from marine sediment of the South China Sea[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2015,65:3384-3391.
APA	Pan, Hua-Qi.,Zhang, Dao-Feng.,Li, Li.,Jiang, Zhao.,Cheng, Juan.,...&Li, Wen-Jun.(2015).Nocardiopsis oceani sp nov and Nocardiopsis nanhaiensis sp nov., actinomycetes isolated from marine sediment of the South China Sea.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,65,3384-3391.
MLA	Pan, Hua-Qi,et al."Nocardiopsis oceani sp nov and Nocardiopsis nanhaiensis sp nov., actinomycetes isolated from marine sediment of the South China Sea".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65(2015):3384-3391.